Cytoskeleton dynamics: Fluctuations within the network
Cytoskeleton dynamics: Fluctuations within the network
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DOI：
10.1016/j.bbrc.2007.01.191
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发表时间：
2007-04-06
影响因子：
3.1
通讯作者：
An, Steven S.
中科院分区：
文献类型：
--
作者：
Bursac, Predrag;Fabry, Ben;An, Steven S.
非平衡系统，例如活细胞骨架 (CSK) 的动力学，本质上是嘈杂的，并且由于潜在的生化和分子事件的随机性质而产生波动。最近，通过观察与细胞表面结合的 RGD 涂层微珠的自发纳米级运动来表征细胞内的这种波动 [Bursae 等人，Nat.马特。 4（2005）557-561]。虽然这些报道的异常珠运动代表了与珠接触的微结构的分子水平重组（重塑），但这些细胞骨架成分和驱动其重塑动态的力的精确性质在很大程度上尚不清楚。在这里，我们重点关注 RGD 包被珠子的自发运动，特别是评估这些运动在多大程度上归因于 (i) 大量细胞运动（细胞爬行）、(ii) 粘着斑动力学、(iii) 脂质膜动力学和/或 (iv) 由肌球蛋白马达驱动的基础肌动蛋白 CSK 的动力学。 (c) 2007 Elsevier Inc. 保留所有权利。
Out-of-equilibrium systems, such as the dynamics of a living cytoskeleton (CSK), are inherently noisy with fluctuations arising from the stochastic nature of the underlying biochemical and molecular events. Recently, such fluctuations within the cell were characterized by observing spontaneous nano-scale motions of an RGD-coated microbead bound to the cell surface [Bursae et al., Nat. Mater. 4 (2005) 557-561]. While these reported anomalous bead motions represent a molecular level reorganization (remodeling) of microstructures in contact with the bead, a precise nature of these cytoskeletal constituents and forces that drive their remodeling dynamics are largely unclear. Here, we focused upon spontaneous motions of an RGD-coated bead and, in particular, assessed to what extent these motions are attributable to (i) bulk cell movement (cell crawling), (ii) dynamics of focal adhesions, (iii) dynamics of lipid membrane, and/or (iv) dynamics of the underlying actin CSK driven by myosin motors. (c) 2007 Elsevier Inc. All rights reserved.