Molecular diversity of the Planctomycetes in the human gut microbiota in France and Senegal
Molecular diversity of the Planctomycetes in the human gut microbiota in France and Senegal
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DOI：
10.1111/apm.12087
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发表时间：
2013-11-01
期刊：
影响因子：
2.8
通讯作者：
Drancourt, Michel
中科院分区：
文献类型：
--
作者：
Cayrou, Caroline;Sambe, Bissoume;Drancourt, Michel
到目前为止，植物菌被认为是一种环境生物。尽管如此，一些研究从人类肠道中检测到了plantometes DNA。因此，我们探索了人类肠道中植物菌的含量。设计plantomytes特异性PCR引物，扩增来自法国和塞内加尔个体以及法国接受抗生素治疗的心内膜炎患者的粪便标本中240 bp的16S rRNA基因片段。然后对PCR产物进行克隆和测序。PCR检测结果显示，塞内加尔粪便标本中plantomycetes 16S rRNA基因的检出率（1.8% vs 0.4%, p=0.05）和多样性（62 vs 6种，p=0.02）显著高于法国。此外，心内膜炎患者的粪便标本的患病率（0.6% vs 0.4%）和阳性患者的种型比率（3 vs 1.5）也没有明显高于未治疗的法国人。在6/12的个体中发现了Gemmata sp.的相关序列。植物菌是人类消化道微生物群的一部分。它们的多样性因环境而异，包括个体的地理来源和抗生素治疗。
Until now, Planctomycetes bacteria were considered as environmental organisms. Nevertheless, some studies detected Planctomycetes DNA from human gut. We therefore explored the human gut Planctomycetes content. Planctomycetes-specific PCR primers were designed to amplify a 240-bp 16S rRNA gene fragment in human stool specimens from individuals in France and in Senegal and from endocarditis patients receiving antibiotics in France. PCR products were then cloned and sequenced. PCR detection revealed a significantly higher prevalence (1.8% vs 0.4%, p=0.05) and higher diversity (62 vs 6 phylotypes, p=0.02) of Planctomycetes 16S rRNA gene in stool specimens collected in Senegal than in France. Also, stool specimens from endocarditis patients exhibited non-significantly higher prevalence (0.6% vs 0.4%) and the ratio of phylotypes by positive patient (3 vs 1.5) than those collected from untreated French individuals. Gemmata sp. related sequences were found in 6/12 individuals. Planctomycetes organisms are a part of the human digestive tract microbiota. Their diversity varied by environment including the geographical origin of the individual and antibiotics treatment.