Association of Little cherry virus 1 (LChV1) with the Shirofugen Stunt Disease and Characterization of the Genome of a Divergent LChV1 Isolate
Association of Little cherry virus 1 (LChV1) with the Shirofugen Stunt Disease and Characterization of the Genome of a Divergent LChV1 Isolate
复制标题
DOI：
10.1094/phyto-10-12-0275-r
复制
发表时间：
2013-03-01
期刊：
影响因子：
3.2
通讯作者：
Gentit, Pascal
中科院分区：
文献类型：
--
作者：
Candresse, Thierry;Marais, Armelle;Gentit, Pascal
Candresse，T.，Marais，A.，福尔角，and Gentit，P. 2013.小樱桃病毒1（LChV 1）与Shirofugen矮化病的关联和不同LChV 1分离物基因组的表征。植物病理学103：293-298。使用454焦磷酸测序多重方法对从Shirofugen矮化病（SSD）的V2356（“Yelsa”）酸樱桃来源纯化的双链RNA进行测序。将获得的15，646个读段组装成279个重叠群，其中5个，总计近16.9 kbp和5，332个读段（样品读段的34%），显示出高Blast评分和与小樱桃病毒I（LChV 1）的同源性。这五个重叠群进一步手动组装成三个超级重叠群，跨越整个LChV 1基因组，只有两个小缺口（17和55个碱基）。使用靶向聚合酶链反应和从重叠群设计的引物完成病毒基因组的测序。在剩余的重叠群或单例中，未获得V2356来源中存在其他病毒因子的证据。V2356 LChV 1分离株与参考完整LChV 1序列仅约76%相同，特别是与Kwanzan发育迟缓综合征相关的ITMAR分离株。然而，它是高度同源的（97至100%的同一性），在两个短的基因组区域不同的LChV 1从北美，提供了第一个完整的序列，这样的分歧分离。虽然没有提供明确的证据，但未能在V2356 SSD来源中检测到任何其他病毒因子，并且在第二个独立的疾病来源中鉴定出LChV 1，这表明LChV 1分离株可能是SSD综合征的原因。
Candresse, T., Marais, A., Faure, C., and Gentit, P. 2013. Association of Little cherry virus 1 (LChV1) with the shirofugen stunt disease and characterization of the genome of a divergent LChV1 isolate. Phytopathology 103:293-298. Double-stranded RNAs purified from the V2356 ('Successa') sour cherry source of the Shirofugen stunt disease (SSD) were sequenced using a 454 pyrosequencing multiplex approach. The 15,646 reads obtained were assembled into 279 contigs, 5 of which, totaling almost 16.9 kbp and 5,332 reads (34% of sample reads), showed high Blast scores and homology to Little cherry virus I (LChV1). The five contigs were further assembled manually into three supercontigs spanning the full LChV1 genome with only two small gaps (17 and 55 bases). Completion of the sequencing of the viral genome was performed using targeted polymerase chain reaction and primers designed from the contigs. No evidence for the presence of other viral agents in the V2356 source could be obtained in the remaining contigs or singletons. The V2356 LChV1 isolate is only approximate to 76% identical with the reference complete LChV1 sequences and, in particular, with the ITMAR isolate associated with the Kwanzan stunting syndrome. However, it is highly homologous (97 to 100% identity) in two short genome regions with divergent LChV1 from North America, providing the first complete sequence for such divergent isolates. Although not providing a definite proof, the failure to detect any other viral agent in the V2356 SSD source and the identification of LChV1 in a second, independent, source of the disease suggests that LChV1 isolates could be responsible for the SSD syndrome.