Estrogenic activity of natural and synthetic estrogens in human breast cancer cells in culture.
Estrogenic activity of natural and synthetic estrogens in human breast cancer cells in culture.
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DOI：
10.1289/ehp.97105s3637
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发表时间：
1997-04
影响因子：
10.4
通讯作者：
Duwe G
中科院分区：
文献类型：
--
作者：
Zava DT;Blen M;Duwe G
我们研究了各种环境污染物（外源性物质）的雌激素活性，特别是外源性雌激素o，p-DDT，并比较了它们与内源性雌激素，植物雌激素和真菌雌激素对雌激素受体结合能力，诱导雌激素终产物和激活单层培养的雌激素敏感的人乳腺癌细胞增殖的影响。我们还量化了一些常见食物、草药和香料提取物中的植物雌激素水平，以及食用高植物雌激素食物来源（豆奶）后人类唾液中的植物雌激素水平，以比较植物雌激素丰度和生物利用度相对于人类报告的异种雌激素负荷。结果表明，天然内源性雌激素，植物雌激素，真菌雌激素，和异种雌激素结合雌激素受体（ER）在完整的细胞，但表现出显着的差异，他们的能力，以诱导雌激素作用的终产物和调节细胞增殖。所有不同种类的雌激素在半饱和ER的浓度下刺激细胞增殖，但只有某些种类能够诱导雌激素调节的终产物。染料木黄酮是一种常见的植物雌激素，存在于大豆食品中，它与异雌激素DDT的不同之处在于它对细胞增殖的影响和诱导雌激素调节的终产物的能力。此外，我们发现，人类通常食用的许多食物，草药和香料都含有大量的植物雌激素，而食用豆浆，一种富含植物雌激素的食物，会显着增加唾液中植物雌激素的水平。总之，我们的体外结果预测，富含植物雌激素的饮食将显着减少体内靶组织中弱异种雌激素与ER的结合。
We investigated the estrogenic activity of various environmental pollutants (xenobiotics), in particular the xenoestrogen o,p-DDT, and compared their effects with those of endogenous estrogens, phytoestrogens, and mycoestrogens on estrogen receptor binding capacity, induction of estrogen end products, and activation of cell proliferation in estrogen-sensitive human breast cancer cells in monolayer culture. We also quantified the levels of phytoestrogens in extracts of some common foods, herbs, and spices and in human saliva following consumption of a high phytoestrogen food source (soy milk) to compare phytoestrogen abundance and bioavailability relative to the reported xenoestrogen burden in humans. Results show that natural endogenous estrogens, phytoestrogens, mycoestrogens, and xenoestrogens bind estrogen receptor (ER) in intact cells, but demonstrate marked differences in their ability to induce end products of estrogen action and to regulate cell proliferation. All of the different classes of estrogens stimulated cell proliferation at concentrations that half-saturated ER, but only some classes were able to induce estrogen-regulated end products. Genistein, a common phytoestrogen found in soy foods, differed from the xenoestrogen DDT in its effects on cell proliferation and ability to induce estrogen-regulated end products. Moreover, we found that many of the foods, herbs, and spices commonly consumed by humans contain significant amounts of phytoestrogens, and consumption of soy milk, a phytoestrogen-rich food, markedly increases the levels of phytoestrogens in saliva. In conclusion, our in vitro results predict that a diet high in phytoestrogens would significantly reduce the binding of weak xenoestrogens to ER in target tissues in vivo.