Color-imaging histodiagnostic approach for cancer
Color-imaging histodiagnostic approach for cancer
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癌症的彩色成像组织诊断方法
DOI：
10.15761/imm.1000145
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A. Sasaki
中科院分区：
文献类型：
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作者：
A. Sugitachi;K. Otsuka;Toshimoto Kimura;Masanori Hakozaki;M. Yaegashi;Megumu Kamishima;K. Kume;S. Nishizuka;A. Sasaki
本文设计了一种彩色显像方法用于肿瘤组织学诊断，并探讨了其临床应用的可行性和意义。根据光动力学诊断（PDD）理论，通过识别癌细胞在蓝光照射下发出的特定红粉色荧光，在显微镜下检测癌症。氨基乙酰丙酸（ALA）用于处理组织学样品。三种不同类型的肿瘤细胞系被用作肿瘤模型。在体外实验中，我们确定用含有ALA的培养基溶液处理的每个肿瘤细胞在蓝光下发出红色荧光。在临床研究中，将手术期间从癌症患者新鲜采集的标本直接浸泡在含有ALA的培养基中，并在37°C下孵育一定时间。然后在蓝色光束下进行显微镜观察。放射出红粉色荧光的样品被认为是癌症。在37°C下用1%ALA/细胞培养基处理30分钟，每个模型细胞发出粉红色荧光。在相同条件下，非存活和/或凋亡细胞不显示荧光。用ALA培养基溶液处理的临床收获的癌症区域也显示粉红色荧光。我们可以在30-60分钟内鉴别标本的恶变，而无需制备冰冻切片进行快速诊断。这种简单的方法在临床上是一种有用的快速癌症诊断工具。
We contrived a color-imaging manner in histodiagnosis of cancer and evaluated its clinical feasibility and significance. Cancer was microscopically detected by identifying specific reddish pink fluorescence emitted from cancer cells under blue light irradiation, which was based on a photodynamic diagnosis (PDD) theory. Aminolevulinic acid (ALA) was used to treat the histologic samples. Three different types of tumor cell lines were used as neoplastic models. In in vitro, we ascertained that each tumor cell treated with ALA-contained medium solution emitted red fluorescence under blue light. In clinical studies, freshly harvested specimens from cancer patients during surgical operations were directly soaked into ALA-containing culture medium, which were incubated at 37°C for a certain minutes. Then microscopically observed under the blue light beam. The samples radiated reddish pink fluorescence were regarded as cancer. Each model cell emitted pink fluorescence by treating with 1% ALA/cell culture medium at 37°C for 30 min. Non-viable and/or apoptotic cells showed no fluorescence under the same conditions. Clinically harvested cancer regions treated with the ALA-medium solution also showed pink fluorescence. We could discriminate malignant change of the samples within 30-60 minutes without preparing frozen sections for rapid diagnoses. This simple method suggested clinically useful tool in a rapid cancer diagnosis.