ISCHEMIA-INDUCED SHIFT OF INHIBITORY AND EXCITATORY AMINO-ACIDS FROM INTRACELLULAR TO EXTRACELLULAR COMPARTMENTS
ISCHEMIA-INDUCED SHIFT OF INHIBITORY AND EXCITATORY AMINO-ACIDS FROM INTRACELLULAR TO EXTRACELLULAR COMPARTMENTS
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DOI：
10.1038/jcbfm.1985.56
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发表时间：
1985-01-01
影响因子：
6.3
通讯作者：
HAMBERGER, A
中科院分区：
文献类型：
--
作者：
HAGBERG, H;LEHMANN, A;HAMBERGER, A
通过夹闭其椎动脉先前已被电烙的兔的颈总动脉诱导脑缺血10或30分钟。EEG和组织高能磷酸盐含量用于验证缺血状态和评估缺血后恢复的程度。在缺血和再灌注4 h的海马细胞外氨基酸水平和总含量。在30分钟的缺血期结束时，GABA增加了250倍，谷氨酸160倍，天冬氨酸和牛磺酸30倍，在细胞外阶段。再流后30 min内恢复正常。延迟增加的细胞外磷酸乙醇胺和乙醇胺达到高峰后1-2小时的回流。10分钟的局部缺血引起了相当小但相似的影响。相对于总氨基酸在海马，谷氨酸和天冬氨酸下降到30-50%的控制，而GABA出现4小时后的回流不受影响。丙氨酸、缬氨酸、苯丙氨酸、亮氨酸和异亮氨酸增加了几倍。毒性细胞外水平的兴奋性氨基酸，以及高细胞外水平的抑制性氨基酸的重要性，被认为是在脑缺血过程中神经细胞损失的病理生理学。
Brain ischemia was induced for 10 or 30 min by clamping the common carotid arteries in rabbits whose vertebral arteries had previously been electrocauterized. EEG and tissue content of high energy phosphates were used to verify the ischemic state and to evaluate the degree of postischemic recovery. Extracellular levels and total contents of amino acids were followed in the hippocampus during ischemia and 4 h of recirculation. At the end of a 30-min ischemic period, GABA had increased 250 times, glutamate 160 times, and aspartate and taurine 30 times in the extracellular phase. The levels returned to normal within 30 min of reflow. A delayed increase of extracellular phosphoethanolamine and ethanolamine peaked after 1-2 h of reflow. Ten minutes of ischemia elicited considerably smaller but similar effects. With respect to total amino acids in the hippocampus, glutamate and aspartate decreased to 30-50% of control while GABA appeared unaffected after 4 h of reflow. Alanine, valine, phenylalanine, leucine, and isoleucine increased severalfold. The importance of toxic extracellular levels of excitatory amino acids, as well as of high extracellular levels of inhibitory amino acids, are considered in relation to the pathophysiology of neuronal cell loss during cerebral ischemia.