Imaging of jasmonic acid binding sites in tissue
Imaging of jasmonic acid binding sites in tissue
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组织中茉莉酸结合位点的成像
DOI：
10.1016/j.ab.2013.04.032
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发表时间：
2013-09-15
影响因子：
2.9
通讯作者：
Wu, Jian Zhong
中科院分区：
文献类型：
--
作者：
Gao, Yan Hong;Yu, Ying;Wu, Jian Zhong
激素调节植物生长发育、衰老和植物对环境的适应机制;研究植物激素作用的分子机制对于理解这些复杂现象是必要的。然而，对于激素信号转导过程没有可测量的信号。我们合成了一种基于量子点的荧光探针，并应用于植物茉莉酸（JA）结合位点的标记。该标记探针通过使用N-羟基琥珀酰亚胺（NHS）作为偶联剂将巯基乙胺修饰的CdTe量子点与JA偶联而获得。采用透射电子显微镜、动态光散射和荧光光谱对探针CdTe-JA进行表征，并应用于标记绿豆幼苗和拟南芥根尖组织切片中的JA结合位点。激光扫描共聚焦显微镜（LSCM）显示，探针选择性标记JA受体。竞争实验表明，CdTe-JA探针保留了JA原有的生物活性。LSCM三维重建实验表明该探针具有良好的光稳定性。(C)2013 Elsevier Inc. All rights reserved.
Hormones regulate the mechanism of plant growth and development, senescence, and plants' adaptation to the environment; studies of the molecular mechanisms of plant hormone action are necessary for the understanding of these complex phenomena. However, there is no measurable signal for the hormone signal transduction process. We synthesized and applied a quantum dot-based fluorescent probe for the labeling of jasmonic acid (JA) binding sites in plants. This labeling probe was obtained by coupling mercaptoethylamine-modified CdTe quantum dots with JA using N-hydroxysuccinimide (NHS) as a coupling agent. The probe, CdTe-JA, was characterized by transmission electron microscopy, dynamic light scattering, and fluorescent spectrum and applied in labeling JA binding sites in tissue sections of mung bean seedlings and Arabidopsis thaliana root tips. Laser scanning confocal microscopy (LSCM) revealed that the probe selectively labeled JA receptor. The competition assays demonstrated that the CdTe-JA probe retained the original bioactivity of JA. An LSCM three-dimensional reconstruction experiment demonstrated excellent photostability of the probe. (C) 2013 Elsevier Inc. All rights reserved.