IL-1β is a better inducer of apoptosis in human fetal membranes than IL-6
IL-1β is a better inducer of apoptosis in human fetal membranes than IL-6
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DOI：
10.1016/s0143-4004(03)00160-7
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发表时间：
2003-11-01
期刊：
影响因子：
3.8
通讯作者：
Menon, R
中科院分区：
文献类型：
--
作者：
Fortunato, SJ;Menon, R
本研究的目的是比较两种炎症细胞因子（IL-1和IL-6）在早产和早产胎膜早破（pPROM）中升高，以及它们诱导胎膜凋亡的能力。从足月妇女身上收集的胎膜放置在器官移植系统中，用重组人il -1 β和IL-6刺激。采用PCR方法研究促凋亡基因（Fas、FasL、TRADD、FADD）和caspases 2、3、8、9的表达模式。用底物法和TUNEL法分别研究Caspase活性和DNA片段化。Caspase 8和Caspase 9在il -1 β和IL-6处理的羊膜中表达。Caspase 2仅在il -1 β刺激组织中表达。与对照组相比，il -1 β增加了caspase 2、3、8和9的活性，而IL-6处理的膜没有表现出明显的变化。与IL-6治疗相比，il -1 - β治疗后DNA断裂的数量更多。本研究表明，il -1 β比IL-6更能诱导正常人胎膜细胞凋亡。(C) 2003 Elsevier Ltd.版权所有。
The objective of this study was to compare two of the inflammatory cytokines (IL-1 and IL-6) elevated in both preterm labour and preterm premature rupture of the membranes (pPROM), with respect to their ability to induce fetal membrane apoptosis. Fetal membranes collected from women at term were placed in an organ explant system and stimulated with recombinant human IL-1beta and IL-6. The expression patterns of pro-apoptotic genes (Fas, FasL, TRADD, FADD) and caspases 2, 3, 8, 9 were studied using PCR. Caspase activity and DNA fragmentation were studied using substrate assays and TUNEL respectively. Caspase 8 and 9 expressions were induced in IL-1beta and IL-6 treated amniochorion. Caspase 2 expression was seen only in IL-1beta stimulated tissues. When compared to control, IL-1beta increased caspase 2, 3, 8 and 9 activities, whereas IL-6 treated membranes did not exhibit a significant change. DNA fragmentation was seen in greater numbers after IL-1beta treatment than after IL-6 treatment. This study demonstrates that IL-1beta is a better inducer of apoptosis in normal human fetal membranes than IL-6. (C) 2003 Elsevier Ltd. All rights reserved.