Contractile effects of endothelins on isolated ampullar segment of human oviduct in luteal phase of menstrual cycle
Contractile effects of endothelins on isolated ampullar segment of human oviduct in luteal phase of menstrual cycle
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DOI：
10.1016/j.phrs.2008.10.001
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发表时间：
2009-01-01
影响因子：
9.3
通讯作者：
Stefanovic, Srdjan
中科院分区：
文献类型：
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作者：
Jankovic, Slobadan M.;Jankovic, Snezana V.;Stefanovic, Srdjan
内皮素-1通过作用于ETA受体，在月经周期的卵泡期诱导人输卵管壶腹段收缩。本研究的目的是探讨内皮素-1、内皮素-2和内皮素-3对月经周期黄体期患者输卵管壶腹段的影响。由于广泛的子宫肌瘤，20例女性患者在腹式子宫切除和附件切除术中取输卵管(每例一管)。输卵管壶腹纵向放置在器皿中，用等距换能器记录离体液的张力。ET-1使离体壶腹段收缩呈浓度依赖性(EC50=6.80+/-1.2×10(-10)M)，并呈浓度依赖性抑制其节律性收缩(EC50=7.86+/-2.3×10(-10)M)。内皮素-2使离体壶腹段呈浓度依赖性的紧张性收缩(EC50=4.56+/-0.3×10-(10)M)，但不影响其节律性收缩。内皮素-3不影响分离制剂的音调或节律性收缩。ETA受体亚型选择性拮抗剂BQ 123对内皮素-1的抑制作用(pA(2)=9.50)和自发性节律收缩的抑制作用(pA(2)=10.73)，而ETB受体亚型的选择性拮抗剂BQ 788仅抑制内皮素-1的自发性节律收缩作用(pA(2)=9.61)，而对自发性节律收缩的作用不受影响。我们的研究结果表明，在黄体期，ETA和ETB受体都调节张力，只有ETA受体调节人输卵管壶腹部节律性活动。(C)2008爱思唯尔有限公司。保留所有权利。
Endothelin-1 induces contractions of human oviduct ampullar segment in follicular phase of menstrual cycle, acting on ETA receptors. The aim of our study was to investigate effects of endothelin-1, endothelin-2 and endothelin-3 on isolated ampullar segment of human oviducts, taken from the patients in luteal phase of menstrual cycle. Fallopian tubes were taken from 20 female patients (one tube from each patient) during abdominal hysterectomy with adnexectomy, due to extensive uterine fibroids. The oviduct ampulla was mounted in an organ bath longitudinally, and the tension of the isolated preparation was recorded with the isometric transducer. Endothelin-1 produced concentration-dependent tonic contraction of the isolated ampullar segment (EC50 = 6.80 +/- 1.2 x 10(-10) M), and concentration-de pendent inhibition of its rhythmic contractions (EC50 = 7.86 +/- 2.3 x 10(-10) M). Endothelin-2 produced concentration-dependent tonic contraction of the isolated ampullar segment (EC50 = 4.56 +/- 0.3 x 10-(10) M), without affecting its rhythmic contractions. Endothelin-3 did not affect either tone or rhythmic contractions of the isolated preparations. Selective antagonist for ETA receptor subtype, BQ 123, produced inhibition of endothelin-1 effects on both tone (pA(2) = 9.50) and spontaneous rhythmic contractions (pA(2) = 10.73), while selective antagonist for ETB receptor subtype, BQ 788, produced only inhibition of endothelin-1 effects on tone (pA(2) = 9.61), while the effect of endothelin-1 on spontaneous rhythmic contractions remained unaffected. The results of our study suggest that in the luteal phase both ETA and ETB receptors regulate tone, and only ETA receptors regulate rhythmic activity of human oviduct's ampullar segment. (C) 2008 Elsevier Ltd. All rights reserved.