Plasma membrane poration induced by ultrasound exposure: Implication for drug delivery
Plasma membrane poration induced by ultrasound exposure: Implication for drug delivery
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DOI：
10.1016/j.jconrel.2005.01.007
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发表时间：
2005-05-05
影响因子：
10.8
通讯作者：
Guy, RH
中科院分区：
文献类型：
--
作者：
Mehier-Humbert, S;Bettinger, T;Guy, RH
声孔法是一种在超声造影剂 (UCA) 存在下将药物（包括基因）转移到细胞中的技术。在这项研究中，通过间接分子探测和显微镜观察来表征超声应用产生的孔隙尺寸以及孔隙张开的持续时间。直径达 37 nm 的分子的内化是有效的并且通常具有良好的耐受性；另一方面，共聚焦显微镜显示，当应用声孔时，75 nm 颗粒仅进入少数细胞。一般来说，内化的物种越大，转移越差。使用扫描电子显微镜直接观察声波照射后的孔，由于所用细胞表面存在大量绒毛（MAT B III）以及孔打开持续时间短而受到阻碍。使用红细胞可以更清晰地观察穿孔区域。这项研究（i）证实了声孔作用是一种将大分子输送到细胞中的方法，并且（ii）详细描述了超声诱导细胞膜上瞬时孔的现象。 (c) 2005 Elsevier B.V 保留所有权利。
Sonoporation, in the presence of ultrasound contrast agents (UCA), is a technique that permits the transfer of drugs, including genes, into cells. In this study, the size of the pores created by ultrasound application, and the duration of pore opening, have been characterized via indirect molecular probing and microscopic observation. Internalization of molecules with diameters up to 37 nm was efficient and generally well-tolerated; on the other hand, confocal microscopy revealed that 75 nm particles entered only a few cells when sonoporation was applied. In general, the larger the species to internalize, the poorer the transfer. Direct visualization of pores following insonification, using scanning electron microscopy, was hampered by the presence of numerous villi on the surface of the cells employed (MAT B III), and by the short duration of pore opening. Clearer observations of porated regions were possible using red blood cells. This research (i) confirms that sonoporation is a means with which to achieve macromolecule delivery into cells, and (ii) characterizes in some detail the phenomenon of ultrasound induction of transient pores in the cell membrane. (c) 2005 Elsevier B.V All rights reserved.