Guidance of subcellular tubulogenesis by actin under the control of a synaptotagmin-like protein and Moesin
Guidance of subcellular tubulogenesis by actin under the control of a synaptotagmin-like protein and Moesin
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DOI：
10.1038/ncomms4036
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发表时间：
2014-01-01
影响因子：
16.6
通讯作者：
Leptin, Maria
中科院分区：
文献类型：
--
作者：
JayaNandanan, N.;Mathew, Renjith;Leptin, Maria
许多极化上皮细胞的顶膜表现出特殊的形态适应，以实现不同的生理功能。果蝇气管末端细胞的空气输送小管是膜特化的一个极端例子。在这里，我们发现Bitesize (Btsz)，一个突触他敏样蛋白家族成员，是管腔膜形态发生所必需的。在多细胞管和其他上皮中，Btsz通过影响粘附体连接来影响根尖完整性，而在这里，Btsz作用于远离连接的地方。它通过串联C2结构域定位于管腔膜，招募活化的Moesin。这两种蛋白对于管腔膜上肌动蛋白细胞骨架的完整性都是必需的，但对于细胞中其他的f -肌动蛋白池则不需要，对于外膜上的肌动蛋白依赖过程（如丝状活性或膜生长依赖于Btsz）也不需要。Btsz和Moesin通过组织肌动蛋白引导管腔膜的形态发生，并允许含有顶端决定因子的膜的结合。
Apical membranes in many polarized epithelial cells show specialized morphological adaptations that fulfil distinct physiological functions. The air-transporting tubules of Drosophila tracheal terminal cells represent an extreme case of membrane specialization. Here we show that Bitesize (Btsz), a synaptotagmin-like protein family member, is needed for luminal membrane morphogenesis. Unlike in multicellular tubes and other epithelia, where it influences apical integrity by affecting adherens junctions, Btsz here acts at a distance from junctions. Localized at the luminal membrane through its tandem C2 domain, it recruits activated Moesin. Both proteins are needed for the integrity of the actin cytoskeleton at the luminal membrane, but not for other pools of F-actin in the cell, nor do actin-dependent processes at the outer membrane, such as filopodial activity or membrane growth depend on Btsz. Btsz and Moesin guide luminal membrane morphogenesis through organizing actin and allowing the incorporation of membrane containing the apical determinant Crumbs.