Oral DNA vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), against infectious haematopoietic necrosis virus using PLGA [Poly(D,L-Lactic-Co-Glycolic Acid)] nanoparticles
Oral DNA vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), against infectious haematopoietic necrosis virus using PLGA [Poly(D,L-Lactic-Co-Glycolic Acid)] nanoparticles
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DOI：
10.1111/j.1365-2761.2011.01338.x
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发表时间：
2012-03-01
影响因子：
2.5
通讯作者：
Sheridan, P. P.
中科院分区：
文献类型：
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作者：
Adomako, M.;St-Hilaire, S.;Sheridan, P. P.
一种抗传染性造血坏死病毒（IHNV）的DNA疫苗可以有效地保护虹鳟鱼（Oncorhynchus mykiss）免受疾病的侵害，但需要进行肌肉注射，这使得该疫苗不适合用于淡水虹鳟鱼养殖业。聚（D， l -乳酸-羟基乙酸）（PLGA）是美国食品和药物管理局（FDA）批准的可用于递送DNA疫苗的聚合物。我们评估了添加到鱼食颗粒中含有香豆素-6的PLGA纳米颗粒的体内吸收。我们证明了虹鳟鱼会吃PLGA纳米颗粒包裹的饲料，并且这些纳米颗粒可以在喂食后96小时内在下肠上皮细胞中检测到。我们还发现，当鱼被喂食或插管含有IHNV G基因质粒的PLGA纳米颗粒时，基因表达和抗IHNV中和抗体水平较低。一项病毒攻毒评估表明，在接种疫苗后6周，接受高剂量口服疫苗的鱼的存活率略有增加，但在接种疫苗后10周，接受额外攻毒的鱼没有差异。这项研究的结果表明，使用口服DNA疫苗诱导免疫反应是可能的，但目前的系统需要改进。
A DNA vaccine against infectious haematopoietic necrosis virus (IHNV) is effective at protecting rainbow trout, Oncorhynchus mykiss, against disease, but intramuscular injection is required and makes the vaccine impractical for use in the freshwater rainbow trout farming industry. Poly (D,L-lactic-co-glycolic acid) (PLGA) is a U.S. Food and Drug Administration (FDA) approved polymer that can be used to deliver DNA vaccines. We evaluated the in vivo absorption of PLGA nanoparticles containing coumarin-6 when added to a fish food pellet. We demonstrated that rainbow trout will eat PLGA nanoparticle coated feed and that these nanoparticles can be detected in the epithelial cells of the lower intestine within 96 h after feeding. We also detected low levels of gene expression and anti-IHNV neutralizing antibodies when fish were fed or intubated with PLGA nanoparticles containing IHNV G gene plasmid. A virus challenge evaluation suggested a slight increase in survival at 6 weeks post-vaccination in fish that received a high dose of the oral vaccine, but there was no difference when additional fish were challenged at 10 weeks post-vaccination. The results of this study suggest that it is possible to induce an immune response using an orally delivered DNA vaccine, but the current system needs improvement.