Lipopolysaccharide inhibits activation of latent transforming growth factor‐β in bovine endothelial cells
Lipopolysaccharide inhibits activation of latent transforming growth factor‐β in bovine endothelial cells
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脂多糖抑制牛内皮细胞潜在转化生长因子-β的激活
DOI：
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发表时间：
1995
影响因子：
5.6
通讯作者：
D. Rifkin
中科院分区：
文献类型：
--
作者：
S. Kojima;Robert A. Vernooy;D. Moscatelli;H. Amanuma;D. Rifkin
血管内皮细胞（EC）对潜在转化生长因子-β（TGF-β）的激活受细胞纤溶酶原激活物（PA）/纤溶酶、转氨酶（TGase）和潜在TGF-β水平的调节。由于脂多糖（LPS）已被报道通过增加PA抑制剂PA抑制剂-1（PAI-1）的产生来降低EC表面纤溶酶水平，因此我们使用两种不同的系统（即与平滑肌细胞（SMC）共培养的牛主动脉EC（BAEC）和用视黄醇处理的BAEC）来测试LPS是否可能抑制EC中潜伏的TGF-β活化。将BAEC与经15 ng/ml LPS处理后的SMC共培养，或用2 μ M视黄醇和/或10 ng/ml LPS处理，并测量PA、表面纤溶酶、TGase的表达以及分泌到培养物中的活性和潜伏性TGF-β的量。用LPS下调表面PA/纤溶酶水平伴随着TGase和潜伏TGF-β表达的显著下降以及对潜伏TGF-β表面活化的抑制。该作用依赖于LPS的浓度和处理时间。在LPS污染的培养基中维持的细胞中未发生TGF-β的形成。© 1995 Wiley利斯公司
The activation of latent transforming growth factor‐β (TGF‐β) by vascular endothelial cells (ECs) is regulated by cellular plasminogen activator (PA)/plasmin, transglutaminase (TGase), and latent TGF‐β levels. Because lipopolysaccharide (LPS) has been reported to reduce EC surface plasmin levels by increasing the production of the inhibitor of PA, PA inhibitor‐1 (PAI‐1), we have tested whether LPS might suppress latent TGF‐β activation in ECs using two different systems, namely, bovine aortic ECs (BAECs) cocultured with smooth muscle cells (SMCs) and BAECs treated with retinol. BAECs were either cocultured with SMCs after treatment with 15 ng/ml LPS or were treated with 2 μM retinol and/or 10 ng/ml LPS, and the expression of PA, surface plasmin, TGase, and the amounts of active and latent TGF‐β secreted into the culture modium were measured. The downregulation of surface PA/plasmin levels with LPS was accompanied by a profound decline of both TGase and latent TGF‐β expression as well as the suppression of surface activation of latent TGF‐β. The effect was dependent on the concentration of LPS and on treatment time. The formation of TGF‐β did not occur in cells maintained in LPS‐contaminated culture medium. © 1995 Wiley‐Liss, Inc.
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DOI：
10.1073/pnas.88.2.580
发表时间：
1991-01-01
影响因子：
11.1
作者：
DENNIS, PA;RIFKIN, DB
通讯作者：
RIFKIN, DB
培养牛主动脉内皮细胞中 1 型纤溶酶原激活剂抑制剂基因表达的调节。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sawdey,M;Podor,TJ;Loskutoff,DJ
通讯作者：
Loskutoff,DJ
培养物中扰乱牛主动脉内皮细胞产生前列环素。
影响因子：
20.3
作者：
Nawroth,PP;Stern,DM;Kaplan,KL;Nossel,HL
通讯作者：
Nossel,HL
DOI：
10.1073/pnas.86.12.4544
发表时间：
1989-06-01
影响因子：
11.1
作者：
ANTONELLIORLIDGE, A;SAUNDERS, KB;DAMORE, PA
通讯作者：
DAMORE, PA
单核吞噬细胞中的脂多糖受体和信号转导途径。
影响因子：
--
作者：
Chen,TY;Lei,MG;Suzuki,T;Morrison,DC
通讯作者：
Morrison,DC