Probing membrane proteins using atomic force microscopy
Probing membrane proteins using atomic force microscopy
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DOI：
10.1002/jcb.20753
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发表时间：
2006-04-15
影响因子：
4
通讯作者：
Wang, DH
中科院分区：
文献类型：
--
作者：
Li, GY;Xi, N;Wang, DH
为了深入了解弓生物分子的功能，需要能够成像、传感和驱动单分子的先进技术。原子力显微镜(AFM)将是这些任务的新的潜在工具之一。本文对利用原子力显微镜探测生物分子的技术和努力进行了介绍和综述。讨论了使用功能化AFM针尖表征特定单一受体的最新技术。给出了一个用带功能化针尖的原子力显微镜研究感觉神经细胞表达的血管紧张素II 1型受体的例子。在活细胞中使用原子力显微镜识别和表征特定的膜蛋白的前景被提供。鉴于许多疾病的根源是分子水平的，最好的理解是生物纳米机器发生故障，这些独特技术在基础生物医学研究或临床实践中的前景超出了我们的想象。
To gain insights into bow biological molecules function, advanced technologies enabling imaging, sensing, and actuating single molecules are required. The atomic force microscope (AFM) Would be one of novel potential tools for these tasks. In this study, techniques and efforts using AFM to probe biomolecules are introduced and reviewed. The state-of-art techniques for characterizing specific single receptor using the functionalized AFM tip are discussed. An example of studying the angiotensin II type 1 (AT1) receptors expressed in sensory neuronal cells by AFM with a functionalized tip is given. Perspectives for identifying and characterizing specific individual membrane proteins using AFM in living cells are provided. Given that many diseases have their roots at the molecular Scale and are best Understood as a malfunctioning biological nanomachines, the prospects of these unique techniques in basic biomedical research or in clinical practice are beyond Our imagination.