Characterization of 79 microsatellite DNA markers in the Pacific oyster Crassostrea gigas
Characterization of 79 microsatellite DNA markers in the Pacific oyster Crassostrea gigas
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DOI：
10.1046/j.1471-8286.2003.00406.x
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发表时间：
2003-06-01
期刊：
影响因子：
--
通讯作者：
Hedgecock, D
中科院分区：
文献类型：
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作者：
Li, G;Hubert, S;Hedgecock, D
我们对太平洋牡蛎中的79个微卫星DNA标记进行了表征，这些标记是从富含CA、GA、ATG和TAGA基序重复的基因组库中获得的。对于8个F-1的祖父母或曾祖父母的映射家庭，平均杂合性，0.705，每个位点的等位基因的平均数，5.7，没有不同的图案重复或图案复杂性类别。非扩增PCR无效等位基因，这是由分离的作图家庭确认，检测到41（51.9%）的79个位点。从C. angulata、角毛蕨C. sikamea，C. ariakensis和C. virginica随远离焦点种C.千兆。
We characterized 79 microsatellite DNA markers, which were obtained from genomic libraries enriched for CA, GA, ATG and TAGA motif repeats, in the Pacific oyster Crassostrea gigas. For eight F-1 grandparents or great-grandparents of mapping families; the average heterozygosity, 0.705, and average number of alleles per locus, 5.7, did not vary among motif-repeat or motif-complexity categories. Non-amplifying polymerase chain reaction null alleles, which were confirmed by segregation in the mapping families, were detected at 41 (51.9%) of the 79 loci. Cross-species amplifications from C. angulata, C. sikamea, C. ariakensis and C. virginica showed a precipitous decline with distance from the focal species C. gigas.