Distinct response of human B cell subpopulations in recognition of an innate immune signal, CpG DNA
Distinct response of human B cell subpopulations in recognition of an innate immune signal, CpG DNA
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DOI：
10.4049/jimmunol.169.5.2368
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发表时间：
2002-09-01
影响因子：
4.4
通讯作者：
Choi, YS
中科院分区：
文献类型：
--
作者：
Jung, J;Yi, AK;Choi, YS
天然免疫最近重新引起了人们对其调节适应性免疫能力的兴趣。在先天免疫信号中，CpG DNA已显示其作为疫苗佐剂的潜力。然而，CpG DNA对体液免疫应答的影响的细胞机制还不清楚。在这里，我们研究了CpG DNA对人类B细胞分化的影响，使用高度纯化的B细胞亚群：幼稚，生发中心（GC），记忆B细胞。在模拟体内初次或二次免疫应答的体外培养系统中，CpG DNA显著增强了幼稚和记忆B细胞的增殖和浆细胞的生成。CpG DNA显著增加GC B细胞的浆细胞生成。然而，CpG DNA对GC B细胞产生记忆B细胞没有影响。这些结果表明，CpG DNA通过增强终末分化而增强B细胞的适应性免疫应答，但不影响记忆B细胞的产生。
Innate immunity has recently gained renewed interest in its ability to regulate adaptive immunity. Among the innate immune signals, CpG DNA has revealed its potential as a vaccine adjuvant. However, the cellular mechanism for the effect of CpG DNA on the humoral immune response is not well understood. Here, we investigated the effects of CpG DNA on human B cell differentiation using highly purified B cell subsets: naive, germinal center (GC), and memory B cells. In the in vitro culture system that mimics the primary or secondary immune response in vivo, CpG DNA markedly augmented the proliferation and generation of plasma cells from naive and memory B cells. CpG DNA dramatically increased plasma cell generation from GC B cells. However, CpG DNA did not have effect on memory B cell generation from GC B cells. These results suggest that CpG DNA potentiates the B cell adaptive immune response by enhancing terminal differentiation, but does not affect the generation of memory B cells.