Nitrogen addition alters ectomycorrhizal fungal communities and soil enzyme activities in a tropical montane forest
Nitrogen addition alters ectomycorrhizal fungal communities and soil enzyme activities in a tropical montane forest
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DOI：
10.1016/j.funeco.2017.02.004
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发表时间：
2017-06-01
期刊：
影响因子：
2.9
通讯作者：
Dalling, James W.
中科院分区：
文献类型：
--
作者：
Corrales, Adriana;Turner, Benjamin L.;Dalling, James W.
人为氮沉降导致的氮素有效性的长期增加会强烈影响外生菌根真菌群落，并对养分循环、森林组成和碳储量产生潜在影响。在巴拿马的热带山地森林中，我们使用Illumina扩增子测序来研究9 y的实验N添加如何影响与Oreomunnea mexicana相关的EM群落，并通过荧光测定来测量土壤中酶活性的变化。氮的添加显着减少EM定植的Oreomunnea根和改变EM组成。随着氮素添加量的增加，蜡蘑属和乳菇属的丰度增加，而丝膜菌属的丰度下降。此外，我们发现土壤磷酸酶，N-乙酰氨基葡萄糖苷酶，和木聚糖酶的活性与N此外减少。我们的结论是，减少EM真菌类群专门在有机N和磷的吸收沿着减少EM定植的宿主植物可以降低土壤酶活性，因此对土壤养分循环的反馈效应。(C)2017 Elsevier Ltd和英国真菌学会。All rights reserved.
Long-term increases in nitrogen (N) availability resulting from anthropogenic N deposition can strongly influence ectomycorrhizal (EM) fungal communities with potential consequences for nutrient cycling, forest composition and carbon storage. In a tropical montane forest in Panama, we used Illumina amplicon sequencing to examine how 9 y of experimental N addition has affected EM communities associated with Oreomunnea mexicana, and fluorescence assays to measure changes in enzyme activity in the soil. Nitrogen addition significantly reduced EM colonization of Oreomunnea roots and altered EM composition. The abundance of Laccaria and Lactarius increased with N addition, while Cortinarius declined. In addition, we found a reduction in soil phosphatase, N-acetyl-glucosaminidase, and fl-xylanase activity with N addition. We conclude that a reduction in EM fungal taxa specialized in organic N and phosphorus absorption along with a decrease in EM colonization of host plants could decrease soil enzyme activity and therefore have feedback effects on soil nutrient cycling. (C) 2017 Elsevier Ltd and British Mycological Society. All rights reserved.