The effect of storage conditions on salivary cortisol concentrations using an Enzyme Immunoassay
The effect of storage conditions on salivary cortisol concentrations using an Enzyme Immunoassay
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DOI：
10.3109/00365513.2014.985252
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发表时间：
2015-01-01
影响因子：
2.1
通讯作者：
Frokjaer, Vibe G.
中科院分区：
文献类型：
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作者：
Nalla, Anjana A.;Thomsen, Gerda;Frokjaer, Vibe G.
唾液样本很容易收集，并适用于家庭采样，例如，当研究HPA轴动力学，以表征昼夜皮质醇配置文件和皮质醇觉醒反应。然而，储存和运输条件可能是家庭采样方法的关键。在这里，我们测试了唾液皮质醇在不同温度下储存的样品中的稳定性，并在反复解冻-冷冻循环后，用酶免疫测定法（EIA）进行测量。13名健康志愿者，6名女性和7名男性，平均年龄31岁（范围26-49），在早晨（08：00-10：00 h）或午餐前（11：00-12：00 h）收集唾液。测试了在六种不同条件下的储存：在-18 ℃、-4 ℃、4 ℃和室温下储存72 h。测试的一个条件是在-18 ° C下保持72小时，然后在室温环境中在具有冷冻元件的封套中保持72小时，之后将其储存在-80 ° C下。最后一管直接储存在-80摄氏度，并作为“黄金标准”。使用唾液皮质醇诊断EIA测定唾液样品。通过单样本t检验评估了五种条件中的每一种与“金标准”（-80摄氏度）之间皮质醇测量值的差异。未观察到显著差异。这表明，EIA方法可以可靠地用于测量唾液皮质醇样品时，通过家庭采样，包括邮政递送。
Saliva samples are easy to collect and are applicable for home-sampling, e.g. when studying HPA-axis dynamics to characterize diurnal cortisol profiles and the cortisol awakening response. However, the storing and transport conditions might be critical in the home-sampling approach. Here, we tested the stability of saliva cortisol in samples stored at different temperatures and after repeated thawing-freezing cycles when measured with an Enzyme Immuno Assay (EIA). Thirteen healthy volunteers, six women and seven men, mean age 31 (range 26-49) years collected saliva either in the morning hours (08:00-10:00 h) or before lunch (11:00-12:00 h). Storage at six different conditions were tested: Storage at -18 degrees C, -4 degrees C, 4 degrees C and room temperature for 72 h. One condition tested was at -18 degrees C for 72 h and then kept in an envelope for 72 h with a freezing element in room temperature surroundings where after it was stored at -80 degrees C. The last tube was stored directly at -80 degrees C and served as the 'gold standard'. The saliva samples were assayed using Salivary Cortisol Diagnostic EIA. Differences in cortisol measurements between each of the five conditions and the 'gold standard' (-80 degrees C) were evaluated by one-sample t-test. No significant differences were observed. This indicates that an EIA method can be used reliably when measuring salivary cortisol samples obtained by home-sampling including a postal delivery.