Effects of acclimation and pH on ammonia inhibition for mesophilic methanogenic microflora
Effects of acclimation and pH on ammonia inhibition for mesophilic methanogenic microflora
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DOI：
10.1016/j.wasman.2018.09.016
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发表时间：
2018-10-01
期刊：
影响因子：
8.1
通讯作者：
Kim, Sang-Hyoun
中科院分区：
文献类型：
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作者：
Park, Jong-Hun;Yoon, Jeong-Jun;Kim, Sang-Hyoun
这项工作评估了氨对嗜温产甲烷菌群的毒性，相对于驯化和pH值。微生物驯化总氨氮（TAN）浓度为1.0-2.5克N/L和中性至碱性pH值，7.5-8.0，用于厌氧毒性试验（ATA）与TAN高达6.1和pH值范围从7至8.2。采用非竞争模型描述了氨对比产甲烷活性和甲烷产率的影响。驯化提高了产甲烷菌群对氨的耐受性。在pH值范围内，各TAN浓度下的SMA和MY值无显著差异，但游离氨氮（FAN）浓度沿着升高，这意味着TAN而不是FAN将是氨抑制的有用标准。丙酸降解更抑制相比，乙酸裂解产甲烷氨。在pH 7.4时，TAN对醋酸盐的SMA、丙酸盐的SMA、醋酸盐的MY和丙酸盐的MY的半最大抑制浓度（IC 50）分别为3.42-4.26、3.14-3.91、3.67-4.07和3.34-3.88 g N/L。454-对接种物的焦磷酸测序分析表明，古菌群落以甲烷杆菌科和甲烷杆菌科为主，这两种菌被认为对氨抑制具有耐受性。(C)2018爱思唯尔有限公司版权所有
This work evaluated the toxicity of ammonia toward mesophilic methanogenic microflora, with respect to the acclimation and pH. Microflora acclimated to total ammonia nitrogen (TAN) concentrations of 1.0-2.5 g N/L and neutral to alkaline pH, 7.5-8.0, were used for anaerobic toxicity assays (ATA) with TAN up to 6.1 and pH ranged from 7 to 8.2. The effect of ammonia on the specific methanogenic activity (SMA) and methane yield (MY) was described using a noncompetitive model. Acclimation increased the tolerance of the methanogenic microflora to ammonia over the acclimation range. There was no significant difference in the values of SMA and MY at each TAN concentration in the examined pH range, although free ammonia nitrogen (FAN) concentrations increased along with pH. It implied that TAN rather than FAN would be the useful criterion for ammonia inhibition. Propionate degradation was more inhibited compared to acetoclastic methanogenesis by ammonia. The half-maximal inhibitory concentrations (IC50) of TAN for the SMA from acetate, SMA from propionate, MY from acetate, and MY from propionate were 3.42-4.26, 3.14-3.91, 3.67-4.07, and 3.34-3.88 g N/L, respectively, at pH 7.4. 454-pyrosequencing analysis of the inoculum showed that the archaeal community was dominant of Methanosarcinaceae and Methanobacteriaceae, which were known as tolerant to ammonia inhibition. (C) 2018 Elsevier Ltd. All rights reserved.