In vitro selection of DNA to chloroaromatics using magnetic microbead-based affinity separation and fluorescence detection
In vitro selection of DNA to chloroaromatics using magnetic microbead-based affinity separation and fluorescence detection
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DOI：
10.1006/bbrc.1997.6517
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发表时间：
1997-05-08
影响因子：
3.1
通讯作者：
Bruno, JG
中科院分区：
文献类型：
--
作者：
Bruno, JG
采用一种新的方法，利用带有连接臂的磁珠（MBs）对氯代芳烃4-氯苯胺（4-CA）、2，4，6-三氯苯胺（TCA）和五氯苯酚（PCP）的DNA配体进行体外选择（SELEX）。MB的使用有利于避免DNA的洗脱和沉淀，因为具有表面捕获的模板DNA的缀合MB可以直接添加到PCR混合物中。此外，一个简化的PCR方案，只需要一种类型的引物和快速荧光显微镜方法评估核酸结合后，每轮的SELEX证明。(C)北京：科学出版社.
In vitro selection (SELEX) of DNA ligands to the chloroaromatics, 4-chloroaniline (4-CA), 2,4,6-trichloroaniline (TCA) and pentachlorophenol (PCP), was performed by a novel method utilizing magnetic beads (MBs) having a linker arm for immobilization. Use of MBs was advantageous in obviating elution and precipitation of DNA as conjugated MBs with surface-captured template DNA could be directly added to PCR mixtures. In addition, a simplified PCR scheme requiring only one type of primer and a rapid fluorescence microscopic method for assessing nucleic acid binding after each round of SELEX were demonstrated. (C) 1997 Academic Press.