Induction of H‐2‐specific antibodies by injections of syngeneic Sendai virus‐coated cells
Induction of H‐2‐specific antibodies by injections of syngeneic Sendai virus‐coated cells
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通过注射同源仙台病毒包被细胞诱导 H-2 特异性抗体
DOI：
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发表时间：
1987
影响因子：
5.4
通讯作者：
P. Iványi
中科院分区：
文献类型：
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作者：
F. Kievits;A. Rocca;A. Opolski;Jacqueline Limpens;T. Leupers;Tinie Kloosterman;W. Boerenkamp;M. Pla;P. Iványi
通过对C57BL/6 （B6）小鼠同源仙台病毒包被（SV+）脾细胞的抗体应答，研究了B细胞免疫球蛋白受体识别仙台病毒和H - 2b抗原复合物的能力。几乎所有小鼠都产生异位反应性抗H - 2淋巴细胞毒抗体。相反，这种抗体在注射正常（SV -）细胞或仅注射仙台病毒（SV）的小鼠中非常罕见。诱导的细胞毒性抗体的反应模式是可变的，范围从几乎抗私有到广泛交叉反应的血清型。在H‐2基因型、重组型和突变型小鼠株上的反应结果，以及封盖和免疫沉淀实验表明，细胞毒性抗体直接针对H‐2 I类分子。抗H - 2抗体对SV+靶细胞的结合增强，但吸收实验表明，这不是与细胞表面仙台病毒决定因子或H - 2 + SV分子复合物交叉反应的结果。这一结论得到了观察结果的支持，即同源SV+细胞不是诱导的淋巴细胞毒性抗体的主要目标。我们的研究结果不支持MHC限制性抗病毒抗体的存在，但表明通过注射同源SV包被细胞可诱导抗I类H - 2同种异体抗体。我们提出了一个模型，定期诱导抗H‐2抗体没有故意同种免疫。
The capacity of the B cell immunoglobulin receptor to recognize complexes of Sendai viral and H‐2b antigens was investigated by studying the antibody response to injections of syngeneic Sendai virus‐coated (SV+) spleen cells in C57BL/6 (B6) mice. Almost all mice produced alloreactive anti‐H‐2 lymphocytotoxic antibodies. In contrast, such antibodies were found very exceptionally in mice injected with normal (SV−) cells or with Sendai virus (SV) only. The reaction pattern of the cytotoxic antibodies induced was variable and ranged from almost anti‐private to widely cross‐reactive serotypes. The results of reactions on H‐2‐congenic, ‐recombinant and ‐mutant mouse strains, and of capping and immunoprecipitation experiments showed that the cytotoxic antibodies were directed against H‐2 class I molecules. The anti‐H‐2 antibodies exhibited enhanced binding for SV+ target cells, but absorption experiments showed that this was not the result of cross‐reactions with cell surface Sendai viral determinants or with a molecular complex of H‐2 plus SV. This conclusion was supported by the observation that syngeneic SV+ cells were not the predominant targets for the induced lymphocytotoxic antibodies. Our results do not support the existence of MHC‐restricted antiviral antibodies, but show the induction of anti‐class I H‐2 alloantibodies by injections with syngeneic SV‐coated cells. We present a model for regular induction of anti‐H‐2 antibodies without intentional alloimmunization.
小鼠急性病毒感染诱导同种异体反应性细胞毒性 T 细胞。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Yang,H;Welsh,RM
通讯作者：
Welsh,RM