Cancellous Bone Formation Response to Simulated Resistance Training During Disuse Is Blunted by Concurrent Alendronate Treatment
Cancellous Bone Formation Response to Simulated Resistance Training During Disuse Is Blunted by Concurrent Alendronate Treatment
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DOI：
10.1002/jbmr.407
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发表时间：
2011-09-01
影响因子：
6.2
通讯作者：
Bloomfield, Susan A.
中科院分区：
文献类型：
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作者：
Swift, Joshua M.;Swift, Sibyl N.;Bloomfield, Susan A.
本研究的目的是评估模拟阻力训练(SRT)联合阿伦磷酸钠(Alen)在减轻或防止废弃相关松质骨微结构和形成的丢失方面的有效性。将60只6月龄雄性SD大鼠随机分为笼养对照组(CC)、后肢卸载组(HU)、HU+Alen组(HU+Alen)、SRT组(HU+SRT)和Alen+SRT/Alen组(HU+SRT/Alen)。HU+SRT和HU+SRT/Alen大鼠麻醉后，每3天进行一次肌肉收缩(4组5次重复，1000ms等长+1000ms偏心)。另外，HU+Alen和HU+SRT/Alen大鼠每周三次给予Alen 10mug/kg体重。HU使松质骨形成率(BFR)降低了80%，而Alen治疗无效(与CC相比，BFR降低了85%)。HU期间SRT显著增加松质骨BFR，比CC增加123%，而HU+SRT/Alen抑制SRT的合成代谢效应(-70%比HU+SRT)。与CC组相比，SRT组骨体积和骨小梁厚度分别增加19%和9%。此外，HU+SRT组大鼠的类骨表面(OS/BS)显著大于CC组(+32%)。与CC相比，在HU期间向SRT添加Alen降低了Oc.S/BS(-75%)、Ob.S/BS(-72%)、OS/BS(-61%)和血清TRACP5b(-36%)。SRT和Alen各自独立地抑制HU所证实的近两倍的脂肪细胞数量的增加，并抑制骨细胞凋亡的增加。这些结果证明了废用过程中少量高强度肌肉收缩的合成代谢效应，并表明SRT与双膦酸盐联合治疗时，骨吸收和骨形成都受到抑制。(C)2011年美国骨与矿物研究学会。
The purpose of this study was to assess the effectiveness of simulated resistance training (SRT) exercise combined with alendronate (ALEN) in mitigating or preventing disuse-associated losses in cancellous bone microarchitecture and formation. Sixty male Sprague-Dawley rats (6 months old) were randomly assigned to either cage control (CC), hind limb unloading (HU), HU plus either ALEN (HU + ALEN), SRT (HU + SRT), or a combination of ALEN and SRT (HU + SRT/ALEN) for 28 days. HU + SRT and HU + SRT/ALEN rats were anesthetized and subjected to muscle contractions once every 3 days during HU (four sets of five repetitions, 1000 ms isometric + 1000 ms eccentric). Additionally, HU + ALEN and HU + SRT/ALEN rats received 10 mu g/kg of body weight of ALEN three times per week. HU reduced cancellous bone-formation rate (BFR) by 80%, with no effect of ALEN treatment (-85% versus CC). SRT during HU significantly increased cancellous BFR by 123% versus CC, whereas HU + SRT/ALEN inhibited the anabolic effect of SRT (-70% versus HU + SRT). SRT increased bone volume and trabecular thickness by 19% and 9%, respectively, compared with CC. Additionally, osteoid surface (OS/BS) was significantly greater in HU + SRT rats versus CC (+32%). Adding ALEN to SRT during HU reduced Oc.S/BS (-75%), Ob.S/BS (-72%), OS/BS (-61%), and serum TRACP5b (-36%) versus CC. SRT and ALEN each independently suppressed a nearly twofold increase in adipocyte number evidenced with HU and inhibited increases in osteocyte apoptosis. These results demonstrate the anabolic effect of a low volume of high-intensity muscle contractions during disuse and suggest that both bone resorption and bone formation are suppressed when SRT is combined with bisphosphonate treatment. (C) 2011 American Society for Bone and Mineral Research.