Millimeter scale alignment of magnetic nanoparticle functionalized microtubules in magnetic fields.
Millimeter scale alignment of magnetic nanoparticle functionalized microtubules in magnetic fields.
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DOI：
10.1021/ja055815s
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发表时间：
2005-10
影响因子：
15
通讯作者：
M. Platt;Gayatri Muthukrishnan;W. Hancock;M. E. Williams
中科院分区：
文献类型：
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作者：
M. Platt;Gayatri Muthukrishnan;W. Hancock;M. E. Williams
将直径为 14 nm 的 CoFe2O4 纳米粒子与生物素化微管表面结合，使其能够通过外部施加的小型永磁 NdFeB 磁体的磁场进行操纵。微管在驱动蛋白马达修饰的玻璃表面上选择性地形成共平行阵列的图案，模拟毫米距离内磁场线的方向。磁场同时用于增加微管的表面负载。我们证明，微管磁性功能化后，驱动蛋白运动表面的运动性得以保留，而滑动速度取决于中性亲和素连接体以及磁性纳米粒子的负载水平。
The conjugation of 14 nm diameter CoFe2O4 nanoparticles to the surface of biotinylated microtubules enables their manipulation with externally applied magnetic fields of small, permanent NdFeB magnets. Microtubules are selectively patterned on kinesin motor-modified glass surfaces in coparallel arrays that mimic the orientation of the magnetic field lines over millimeter distances. The magnetic field is simultaneously used to increase surface loading of microtubules. We demonstrate that motility across the kinesin motor surface is retained following magnetic functionalization of the microtubules, while gliding speed is dependent on loading level of the neutravidin linker as well as magnetic nanoparticles.