Effect of 72 Hz pulsed magnetic field exposure on macromolecular synthesis in CCRF-CEM cells.
Effect of 72 Hz pulsed magnetic field exposure on macromolecular synthesis in CCRF-CEM cells.
复制标题
72 Hz 脉冲磁场暴露对 CCRF-CEM 细胞大分子合成的影响。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
L. McChesney
中科院分区：
文献类型：
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作者：
J. L. Phillips;L. McChesney
来自t淋巴母细胞样细胞系CCRF-CEM的细胞在体外暴露于37度72 Hz脉冲的准矩形非对称电磁场中30分钟至24小时。通过加入3h -尿苷评估，RNA合成在暴露30分钟后（相对于对照细胞）增加了2倍，在暴露2小时后达到了相对于对照细胞的最大增加3.2倍。在随后的24小时暴露时间内，观察到前体掺入增加。mRNA的合成与观察到的细胞总RNA相似，但不完全相同。此外，暴露2小时后，蛋白质合成（通过将放射性前体掺入酸沉淀物质来测定）比对照组增加了2.8倍。较长的暴露时间导致24小时后前体掺入指数下降至对照水平的1.1倍。使用染料还原试验，线粒体活性也被发现在24小时的暴露期间增加。未发现电磁场暴露对细胞DNA合成有影响。这些数据与其他记录电磁场暴露对体外大分子合成影响的报告大体一致。
Cells from the T-lymphoblastoid cell line, CCRF-CEM, have been exposed in vitro to a quasirectangular, asymmetric electromagnetic field pulsed at 72 Hz at 37 degrees for periods of 30 min to 24 h. RNA synthesis, assessed by incorporation of 3H-uridine, increased (relative to control cells) 2-fold after 30 min in exposed cells and achieved its greatest increase of 3.2-fold relative to controls after 2 h exposure. Increased precursor incorporation was observed at all subsequent exposure times up to 24 h. Synthesis of mRNA was similar, but not identical to that observed with total cellular RNA. Additionally, protein synthesis, determined by incorporation of radioactive precursor into acid-precipitable material, was increased 2.8-fold, compared to controls, after 2 h exposure. Longer exposure times resulted in an exponential decrease in precursor incorporation to 1.1-times control levels after 24 h. Using a dye reduction assay, mitochondrial activity was also found to be increased over a 24 h exposure period. No effect of electromagnetic field exposure was found on cellular synthesis of DNA. These data are generally consistent with other reports documenting effects of electromagnetic field exposure on macromolecular synthesis in vitro.