A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP.
A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP.
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DOI：
10.1039/c3cc39064k
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发表时间：
2013-03
影响因子：
4.9
通讯作者：
Qingwang Xue;Lei Wang;Wei Jiang
中科院分区：
文献类型：
--
作者：
Qingwang Xue;Lei Wang;Wei Jiang
基于哑铃探针介导的滚环扩增(D-RCA)-响应性G-四链构象，发展了一种新的无标记荧光传感方法，用于NAD(+)和ATP的灵敏和选择性检测。该方法对三磷酸腺苷的检测率为0.5 PM，对NAD(+)的检测率为100 fM，远低于以往报道的生物传感器，具有较高的分辨能力。
A novel label-free fluorescent sensing scheme for sensitive and selective detection of NAD(+) and ATP has been developed based on dumbbell probe-mediated rolling circle amplification (D-RCA)-responsive G-quadruplex formation. This approach can detect 0.5 pM for ATP and 100 fM for NAD(+), much lower than those of previously reported biosensors, and exhibits high discrimination ability.