Cell wall remodeling in woody tung oil tree root xylem contributes to deterrence of the Fusarium wilt pathogen
Cell wall remodeling in woody tung oil tree root xylem contributes to deterrence of the Fusarium wilt pathogen
复制标题
DOI：
10.1016/j.indcrop.2023.116361
复制
发表时间：
2023-04
影响因子：
5.9
通讯作者：
Jia Wang;Yang-Lingzhi Yang-Yang-Lingzhi-Yang-19164650;M. Gao;Liwen Wu;Yunxiao Zhao;Yicun Chen;Yangdong Wang
中科院分区：
文献类型：
--
作者：
Jia Wang;Yang-Lingzhi Yang-Yang-Lingzhi-Yang-19164650;M. Gao;Liwen Wu;Yunxiao Zhao;Yicun Chen;Yangdong Wang
油桐（Vernicia fordii）是以生产独特的桐油而闻名于世的油桐树种，近年来遭受枯萎病的危害.有趣的是，它的姊妹种，木本油桐树（V. montana）对镰刀菌表现出很高的抗性。尖孢菌属fordii（Fof-1）感染。我们推测V.因此，在土壤中接种黑根类植物，可作为防止土源镰刀菌入侵的物理屏障。为了验证这一点，我们调查了V.蒙塔纳和V.对Fof-1的反应。结果表明，病原菌能侵入V. fordibut未能进入V.蒙大拿Fof-1感染后，细胞壁厚度增加，细胞壁厚度增加。褐煤木质部显著增加。在侵染初期木质素含量显著增加，在侵染后期木质素与半纤维素的S：H比值显著增加。褐煤木质部细胞壁。进一步的比较代谢组学分析表明，木质素途径中的一些中间代谢物，包括肉桂酸，东莨菪内酯和香豆素，在V中富集。Fof-1感染后的木质部细胞。与此相一致，inV。montana，KEGG富集结果也支持上调基因在该途径中富集的证据。木质素生物合成途径的枢纽基因在V.进一步选择montana，并使用qRT-PCR进行验证。总之，我们的数据表明，细胞壁的重塑在V。木质部对降低Fof-1侵染起主要作用。这一发现为探索培育抗镰刀菌油桐树的有效策略提供了机会。
Tung oil tree (Vernicia fordii), is renowned for production of unique tung oil, which has been sufferedFusariumwilt disease. Intriguingly, its sister species, woody tung oil trees (V. montana) exhibit high resistance toFusarium. oxysporumf. sp.fordii(Fof-1) infection. We hypothesize that the xylem cells ofV. montanaroots might act as physical barriers to deter invasion of the soil-borneFusariumpathogen. To test this, we investigated the potentially different phenotypic and physiological behaviors of the root xylem cells betweenV. montanaandV. fordiiresponding toFof-1. The result showed that pathogen could invade the root xylem ofV. fordiibut failed to enter the root xylem ofV. montana. AfterFof-1 infection, the cell wall thickness inV. montanaroot xylem was statistically increased. A statistically significant increase of lignin in the early stage, and the ratio of S:H lignin and hemicellulose content in the later infection stage were viewed in theV. montanaroot xylem cell wall. Further comparative metabolomic analysis showed that some intermediary metabolites in the lignin pathways, including cinnamic acid, scopoletin and coumarin, were enriched inV. montanaroot xylem cells afterFof-1 infection. Consistent with this, inV. montana, KEGG enrichment result also support the evidence that the upregulated genes were enriched in this pathway. The hub genes of lignin biosynthesis pathway inV. montanawere further selected and verified using qRT-PCR. Together, our data demonstrated that the remodeling of cell wall inV. montanaroot xylem make primary contributions to decrease theFof-1 infection. Promisingly, the discovery offers an opportunity to explore an effective strategy for breedingFusariumresistant tung oil trees.