In vitro effect of immune serum and bovine granulocytes on juvenile Fasciola hepatica.
In vitro effect of immune serum and bovine granulocytes on juvenile Fasciola hepatica.
复制标题
免疫血清和牛粒细胞对幼年肝片形吸虫的体外作用。
DOI：
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发表时间：
1980
影响因子：
4.6
通讯作者：
D. Franks
中科院分区：
文献类型：
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作者：
W. Duffus;D. Franks
感染了肝片吸虫囊蚴的牛会产生针对刚排出的幼年肝片吸虫的外糖萼的抗体。使用 51 Cr 释放和活力测定，在存在或不存在牛补体的情况下，此类抗体不会对寄生虫造成明显的损害。过量抗体的存在导致抗原-抗体复合物在寄生虫表面形成大量聚集体；这些聚集体最终脱落到介质中。通过选择性刺激小母牛的乳腺获得中性粒细胞和嗜酸性粒细胞，并将其大量附着到涂有 IgG1 或 IgG2 的吸虫上。尽管嗜酸性粒细胞的粘附时间比中性粒细胞的粘附时间更长，但粘附依赖于 Fc 受体。使用 51Cr 释放和活力测定，在抗体依赖性细胞介导的细胞毒性中，使用嗜酸性粒细胞或中性粒细胞，对吸虫没有造成损害；贴壁粒细胞最终脱落。有人认为，幼年吸虫外部糖萼的快速周转和排泄阻止了粒细胞与蠕虫寄生虫的紧密附着，这可能是细胞介导的损伤发生的先决条件。
Cattle, infected with Fasciola hepatica metacercariae, produce antibodies against the outer glycocalyx of freshly excysted juvenile F. hepatica. Using 51Cr-release and viability assays such antibodies in the presence or absence of bovine complement did not cause discernible damage to the parasite. The presence of excess antibody caused the build-up of large aggregates of antigen--antibody complexes over the parasite surface; these aggregates were eventually shed into the medium. Neutrophils and eosinophils were obtained by selective stimulation of the mammary gland of heifers, and attached in large numbers to flukes coated with either IgG1 or IgG2. Attachment was dependent on Fc receptors although the adherence of the eosinophils was more prolonged than that of the neutrophils. Using 51Cr-release and viability assays no damage occurred to the flukes using either eosinophils or neutrophils in antibody-dependent cell-mediated cytotoxicity; adherent granulocytes were eventually shed. It is suggested that the rapid turnover and excretion of the outer glycocalyx of juvenile flukes prevents the intimate attachment of granulocytes to the helminth parasite, which is perhaps a prerequisite for cell-mediated damage to occur.