Woven collagen biotextiles enable mechanically functional rotator cuff tendon regeneration during repair of segmental tendon defects in vivo
Woven collagen biotextiles enable mechanically functional rotator cuff tendon regeneration during repair of segmental tendon defects in vivo
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DOI：
10.1002/jbm.b.34279
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发表时间：
2019-08-01
影响因子：
3.4
通讯作者：
Akkus, Ozan
中科院分区：
文献类型：
--
作者：
Learn, Greg D.;McClellan, Phillip E.;Akkus, Ozan
尽管肩袖修复手术的手术技术和材料有所进步，但初次修复失败仍然很常见。本研究探讨了使用电化学排列胶原（ELAC）线编织成生物纺织品支架作为移植物，以修复新西兰白色兔的严重冈下肌肌腱缺损。评估了三个手术治疗组：接受直接修复的兔作为手术对照，仅接受ELAC支架的兔，以及用间充质干细胞（MSC）接种的ELAC支架治疗的兔。在每只动物中，完整的对侧冈下肌作为内部阳性对照。肌腱-骨结构在体内3个月后收获，结果测量包括生物力学测试、组织学染色和免疫组织化学染色。生物力学测试显示，所有组之间的最大承重能力相当，而MSC接种支架修复相对于非接种支架修复表现出刚度增加。组织学染色显示，与天然肌腱相比，ELAC纤维周围的胶原蛋白沉积强烈，编织支架连续体内的细胞结构增加。免疫组织化学染色显示存在胶原蛋白I和III在所有组中，但前胶原蛋白I和肌腱特异性标记tenomodulin只观察到在接种和非接种ELAC支架修复。这项初步研究的结果保证继续调查ELAC生物织物支架修复关键尺寸的肩袖肌腱缺损。(C)2018年威利期刊公司
Despite advancements in surgical techniques and materials for rotator cuff repair procedures, primary repair failures remain common. This study examines the use of electrochemically aligned collagen (ELAC) threads woven into biotextile scaffolds as grafts to repair critical infraspinatus tendon defects in New Zealand White rabbits. Three surgical treatment groups were evaluated: rabbits undergoing direct repair as operative controls, rabbits receiving ELAC scaffolds alone, and rabbits treated with mesenchymal stem cell (MSC)-seeded ELAC scaffolds. In each animal, the intact, contralateral infraspinatus served as an internal positive control. Tendon-bone constructs were harvested after 3 months in vivo and outcome measures included biomechanical testing, histological staining, and immunohistochemical staining. Biomechanical testing revealed that maximum load-bearing capacity was comparable between all groups, while MSC-seeded scaffold repairs exhibited increased stiffness relative to non-seeded scaffold repairs. Histological staining revealed robust collagen deposition around ELAC fibers and increased cellularity within the continuum of woven scaffolds as compared to native tendon. Immunohistochemical staining revealed presence of collagens I and III in all groups, but procollagen I and the tendon-specific marker tenomodulin were only observed in seeded and non-seeded ELAC scaffold repairs. Findings of this pilot study warrant continued investigation of ELAC biotextile scaffolds for repair of critically-sized rotator cuff tendon defects. (C) 2018 Wiley Periodicals, Inc.