Detergent-resistant Plasma Membrane Proteome in Oat and Rye: Similarities and Dissimilarities between Two Monocotyledonous Plants
Detergent-resistant Plasma Membrane Proteome in Oat and Rye: Similarities and Dissimilarities between Two Monocotyledonous Plants
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DOI：
10.1021/pr200849v
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发表时间：
2012-03-01
影响因子：
4.4
通讯作者：
Uemura, Matsuo
中科院分区：
文献类型：
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作者：
Takahashi, Daisuke;Kawamura, Yukio;Uemura, Matsuo
质膜（PM）参与决定植物细胞生长、发育、分化和环境信号响应的重要细胞过程。这些动态反应中的一些发生在PM中的特定域中。在这项研究中，我们进行了可比的nano-LC-MS/MS为基础的大规模蛋白质组学分析的洗涤剂抗性膜（DRM）组分从燕麦和黑麦的PM。许多蛋白质在PM和DRM之间显示出差异积累，并且一些蛋白质仅在DRM中发现。燕麦和黑麦中有219种蛋白被鉴定为DRM蛋白，其中约一半仅在DRM中被鉴定。DRM蛋白在双子叶植物（拟南芥和烟草）中很大程度上是常见的，这表明植物中与DRM相关的共同功能。结合半定量蛋白质组学分析和翻译后蛋白质修饰位点的预测，揭示了燕麦和黑麦中与DRM相关的几种蛋白质的差异。它的结论是，蛋白质分布在DRM中是独特的，从PM中，部分是因为蛋白质的理化性质，这些蛋白质的独特的分布可能定义的特定域在植物细胞的各种生理过程中的PM的功能。
The plasma membrane (PM) is involved in important cellular processes that determine the growth, development, differentiation, and environmental signal responses of plant cells. Some of these dynamic reactions occur in specific domains in the PM. In this study, we performed comparable nano-LC-MS/MS-based large-scale proteomic analysis of detergent-resistant membrane (DRM) fractions prepared from the PM of oat and rye. A number of proteins showed differential accumulation between the PM and DRM, and some proteins were only found in the DRM. Numerous proteins were identified as DRM proteins in oat (219 proteins) and rye (213 proteins), of which about half were identified only in the DRM. The DRM proteins were largely common to those found in dicotyledonous plants (Arabidopsis and tobacco), which suggests common functions associated with the DRM in plants. Combination of semiquantitative proteomic analysis and prediction of post-translational protein modification sites revealed differences in several proteins associated with the DRM in oat and rye. It is concluded that protein distribution in the DRM is unique from that in the PM, partly because of the physicochemical properties of the proteins, and the unique distribution of these proteins may define the functions of the specific domains in the PM in various physiological processes in plant cells.