Grape Powder Supplementation Prevents Oxidative Stress-Induced Anxiety-Like Behavior, Memory Impairment, and High Blood Pressure in Rats
Grape Powder Supplementation Prevents Oxidative Stress-Induced Anxiety-Like Behavior, Memory Impairment, and High Blood Pressure in Rats
复制标题
DOI：
10.3945/jn.113.174649
复制
发表时间：
2013-06-01
影响因子：
4.2
通讯作者：
Salim, Samina
中科院分区：
文献类型：
--
作者：
Allam, Farida;Dao, An T.;Salim, Samina
我们研究了葡萄粉治疗是否能改善大鼠氧化应激诱导的焦虑样行为、记忆障碍和高血压。采用l -丁硫氨酸（S，R）-亚砜亚胺（BSC）诱导sd大鼠氧化应激。采用四组大鼠：1)对照组（C组，载药注射，并给予自来水），2)葡萄粉处理组（GP组，载药注射，给予15 g/L溶解于自来水中的葡萄粉3周），3)BSO处理组[注射BSO （300 mg/kg体重），ig 7 d，并给予自来水]，4)BSO +葡萄粉处理组（GP+BSO，注射BSO，并给予葡萄粉处理后的自来水）。BSO大鼠焦虑样行为明显高于C、GP大鼠（P < 0.05）。葡萄粉减弱GP+BSO大鼠焦虑样行为。BSO大鼠在短时记忆和长时记忆测试中的错误均明显高于C或GP大鼠（P < 0.05），而GP+BSO大鼠则无此现象。BSO大鼠的收缩压和舒张压明显高于C或GP大鼠（P < 0.05），而葡萄粉对GP+BSO大鼠的高血压有预防作用。此外，脑细胞外信号调节激酶1/2 （ERK-1/2）被激活（P < 0.05），而乙醛酶1 （GLO-1）、谷胱甘肽还原酶1 （GSR-1）、钙/钙调素依赖性蛋白激酶IV型（CAMK-IV）、cAMP反应元件结合蛋白（CREB）和脑源性神经营养因子（BDNF）水平在BSO大鼠中显著低于C或GP大鼠（P < 0.05），而GP+BSO大鼠中无显著差异（P < 0.05）。我们认为葡萄粉通过调节脑ERK-1/2、GLO-1、GSR-1、CAMK-IV、CREB和BDNF水平，可以预防氧化应激诱导的大鼠焦虑、记忆障碍和高血压。
We examined whether or not grape powder treatment ameliorates oxidative stress-induced anxiety-like behavior, memory impairment, and hypertension in rats. Oxidative stress in Sprague-Dawley rats was produced by using L-buthionine(S,R)-sulfoximine (BSC). Four groups of rats were used: 1) control (C; injected with vehicle and provided with tap water), 2) grape powder-treated (GP; injected with vehicle and provided for 3 wk with 15 g/L grape powder dissolved in tap water), 3) BSO-treated [injected with BSO (300 mg/kg body weight), i.p. for 7 d and provided with tap water], and 4) BSO plus grape powder-treated (GP+BSO; injected with BSO and provided with grape powder-treated tap water). Anxiety-like behavior was significantly greater in BSO rats compared with C or GP rats (P < 0.05). Grape powder attenuated BSO-induced anxiety-like behavior in GP+BSO rats. BSO rats made significantly more errors in both short- and long-term memory tests compared with C or GP rats (P < 0.05), which was prevented in GP+BSO rats. Systolic and diastolic blood pressure was significantly greater in BSO rats compared with C or GP rats (P < 0 05), whereas grape powder prevented high blood pressure in GP+BSO rats. Furthermore, brain extracellular signal-regulated kinase-1/2 (ERK-1/2) was activated (P < 0.05), whereas levels of glyoxalase-1 (GLO-1), glutathione reductase-1 (GSR-1), calcium/calmodulin-dependent protein kinase type IV (CAMK-IV), cAMP response element-binding protein (CREB), and brain-derived neurotrophic factor (BDNF) were significantly less (P < 0.05) in BSO but not in GP+BSO rats compared with C or GP rats. We suggest that by regulating brain ERK-1/2, GLO-1, GSR-1, CAMK-IV, CREB, and BDNF levels, grape powder prevents oxidative stress-induced anxiety, memory impairment, and hypertension in rats.