Induction of mRNA for IGF-I and -II during growth hormone-stimulated muscle hypertrophy.
Induction of mRNA for IGF-I and -II during growth hormone-stimulated muscle hypertrophy.
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在生长激素刺激的肌肉肥大过程中诱导 IGF-I 和 -II 的 mRNA。
DOI：
10.1152/ajpendo.1988.255.4.e513
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Bechtel,PJ
中科院分区：
文献类型：
--
作者：
Turner,JD;Rotwein,P;Novakofski,J;Bechtel,PJ
在骨骼肌和心肌肥大过程中胰岛素样生长因子（IGF）基因的表达进行了检查，骨骼肌和心肌肥大使用成年5月龄雌性Wistar-Furth大鼠植入生长激素分泌的GH 3细胞进行了检查。在实验开始后40、60和80天处死对照组和给药组动物。从注射时间到第80天，身体、心脏、骨骼肌和肝脏重量分别增加112、93、55和314%。提取RNA，并使用溶液杂交核酸酶保护试验定量IGF-I和IGF-II mRNA的稳态水平。在对照组织中检测到两种生长因子的mRNA水平较低。到第80天，IGF-I mRNA增加了8倍，IGF-II mRNA增加了6倍。在心肌中，这两种生长因子的mRNA水平上升了三到四倍。虽然生长激素诱导肝脏IGF-I mRNA的增加，IGF-II mRNA仍然几乎检测不到。这项研究表明，在生长过程中，胰岛素样生长因子-I和胰岛素样生长因子-II刺激的肌肉生长的mRNA积累，支持其他观察牵连的胰岛素样生长因子参与骨骼肌生长的旁分泌或自分泌因子。
The expression of insulin-like growth factor (IGF) genes during skeletal and cardiac muscle hypertrophy was examined using skeletal and cardiac muscle hypertrophy was examined using adult 5-mo-old female Wistar-Furth rats implanted with growth hormone-secreting GH3 cells. Control and treated animals were killed at 40, 60, and 80 days after initiation of the experiment. From the time of injection to day 80, body, heart, skeletal muscle, and liver weights increased 112, 93, 55, and 314%, respectively. RNA was extracted and steady-state levels of IGF-I and IGF-II mRNAs were quantitated using a solution-hybridization nuclease-protection assay. Low levels of mRNA for both growth factors were detected in control tissues. By day 80 IGF-I mRNA had increased eightfold and IGF-II mRNA sixfold in skeletal muscle from treated rats. In cardiac muscle the levels of mRNA for both growth factors rose three- to fourfold. Although growth hormone induced an increase in hepatic IGF-I mRNA, IGF-II mRNA remained nearly undetectable. This study shows that during growth hormone-stimulated muscle growth mRNAs for both IGF-I and IGF-II accumulate, supporting other observations implicating the IGFs as paracrine or autocrine factors involved in skeletal muscle growth.