Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
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DOI：
10.1155/2014/208974
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发表时间：
2014-01-01
影响因子：
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通讯作者：
de los Angeles Serradell, Maria
中科院分区：
文献类型：
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作者：
Carasi, Paula;Diaz, Mariangeles;de los Angeles Serradell, Maria
乳酸菌通常被认为是安全的；然而，某些菌株与感染病例有关。我们的工作组已经评估了凯氏乳杆菌的许多功能特性，但在称它们为益生菌之前，必须研究有关安全性的参数。本工作对克氏乳杆菌菌株的安全性和抗菌活性进行了研究。测试的凯氏乳杆菌菌株中没有一种能引起甲型或乙型溶血。所有菌株对四环素、克林霉素、链霉素、氨苄西林、红霉素、卡那霉素、庆大霉素敏感，2株对氯霉素耐药。克氏杆菌对革兰氏(+)和革兰氏(-)病原菌均有抑制作用。对于体外实验结果，选择L.kefii CIDCA 8348进行体内研究。连续21天每天口服10(8)CFU的小鼠没有出现疼痛、嗜睡、脱水或腹泻的迹象，组织学研究与这些发现一致。此外，治疗组和对照组小鼠在促炎细胞因子的分泌方面也没有差异。没有观察到微生物移位到血液、脾或肝脏。关于这些发现，L.kefii CIDCA 8348是一种从乳制品中分离出来的微生物，具有作为人或动物使用的益生菌的巨大潜力。
Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused alpha- or beta- hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(-) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 10(8) CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.