Compound K Production from Red Ginseng Extract by β-Glycosidase from Sulfolobus solfataricus Supplemented with α-L-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus.
Compound K Production from Red Ginseng Extract by β-Glycosidase from Sulfolobus solfataricus Supplemented with α-L-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus.
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DOI：
10.1371/journal.pone.0145876
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Oh DK
中科院分区：
文献类型：
--
作者：
Shin KC;Choi HY;Seo MJ;Oh DK
人参皂苷化合物K（C-K）具有保肝、抗肿瘤、抗衰老、抗皮肤衰老等多种生物活性和药理活性，近年来引起了人们的广泛关注。C-K已被用作护肤品的主要成分。为了使人参提取物有效地应用于化妆品的生产，人参提取物中的PPD型人参皂苷应通过酶转化法转化为C-K。为了提高红参提取物（RGE）中原人参二醇（PPD）型人参皂苷的C-K产率，沿着来自解糖热纤维素酶（CS-abf）的水解α-L-阿拉伯呋喃糖苷的α-L-阿拉伯呋喃糖苷酶和来自硫磺硫化叶菌（Sulfolobus solfataricus）的水解β-D-吡喃葡萄糖苷/α-L-阿拉伯吡喃糖苷的β-糖苷酶（SS-bgly），因为SS-bgly对人参皂苷中α-L-阿拉伯呋喃糖苷键的水解活性非常低。最佳反应条件为：pH 6.0，反应温度80°C，SS-bgly 2 U/mL，CS-abf 3 U/mL，PPD型糖苷7.5 g/L。在此优化条件下，添加CS-abf的SS-bgly在12 h内从7.5 g/L PPD型糖苷合成4.2 g/L C-K，摩尔收率为100%，生产能力为348 mg/L/h。据我们所知，这是文献中公开的人参提取物中C-K的最高浓度和产率。
Ginsenoside compound K (C-K) is attracting a lot of interest because of its biological and pharmaceutical activities, including hepatoprotective, antitumor, anti-wrinkling, and anti-skin aging activities. C-K has been used as the principal ingredient in skin care products. For the effective application of ginseng extracts to the manufacture of cosmetics, the PPD-type ginsenosides in ginseng extracts should be converted to C-K by enzymatic conversion. For increased yield of C-K from the protopanaxadiol (PPD)-type ginsenosides in red-ginseng extract (RGE), the α-l-arabinofuranoside-hydrolyzing α-l-arabinofuranosidase from Caldicellulosiruptor saccharolyticus (CS-abf) was used along with the β-d-glucopyranoside/α-l-arabinopyranoside-hydrolyzing β-glycosidase from Sulfolobus solfataricus (SS-bgly) because SS-bgly showed very low hydrolytic activity on the α-l-arabinofuranoside linkage in ginsenosides. The optimal reaction conditions for C-K production were as follows: pH 6.0, 80°C, 2 U/mL SS-bgly, 3 U/mL CS-abf, and 7.5 g/L PPD-type ginsenosides in RGE. Under these optimized conditions, SS-bgly supplemented with CS-abf produced 4.2 g/L C-K from 7.5 g/L PPD-type ginsenosides in 12 h without other ginsenosides, with a molar yield of 100% and a productivity of 348 mg/L/h. To the best of our knowledge, this is the highest concentration and productivity of C-K from ginseng extract ever published in literature.