Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function

Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
复制标题

DOI:
10.1007/978-1-4939-6820-6_11
复制
发表时间:
2017-01-01
期刊:
THERMOGENIC FAT
影响因子:
--
通讯作者:
Lo, James C.
Lo, James C.
中科院分区:
其他
文献类型:
--
作者:
Gomez-Banoy, Nicolas;Lo, James C.

文献摘要

被引文献

相似文献

病毒载体已成为脂肪组织基因操作的广泛使用的工具,以了解脂肪细胞在代谢中的生物学和功能。有许多不同的病毒载体常用:逆转录病毒,慢病毒,腺病毒和腺相关病毒(AAV)。在这里,我们回顾了文献中的例子,并描述了使用逆转录病毒,慢病毒,腺病毒和AAV来识别脂肪细胞和脂肪组织的方法,以确定脂肪生物学中的基因功能。
Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.