Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
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DOI:
10.1007/978-1-4939-6820-6_11
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发表时间:
2017-01-01
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影响因子:
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通讯作者:
Lo, James C.
中科院分区:
文献类型:
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作者:
Gomez-Banoy, Nicolas;Lo, James C.
Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.