Probiotic strains and their combination inhibit in vitro adhesion of pathogens to pig intestinal mucosa
Probiotic strains and their combination inhibit in vitro adhesion of pathogens to pig intestinal mucosa
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DOI：
10.1007/s00284-007-0144-8
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发表时间：
2007-09-01
影响因子：
2.6
通讯作者：
Salminen, Seppo
中科院分区：
文献类型：
--
作者：
Collado, M. C.;Grzeskowiak, Lukasz;Salminen, Seppo
本研究的目的是调查在体外的商业益生菌菌株（双歧杆菌乳酸Bb12和鼠李糖乳杆菌LGG）单独和组合的保护作用的致病菌株，沙门氏菌，梭菌，大肠杆菌的粘附从不同的肠道区域获得的猪肠道粘液。在组合中，益生菌菌株增强了彼此的粘附，主要是在大肠粘液中。Bb12和LGG单独或联合处理肠粘液，显著降低（P < 0.05）测试病原体的粘附。抑制病原体粘附的能力似乎取决于特定的益生菌和病原体以及粘膜部位。B。lactis Bb12和L.与益生菌菌株相比，组合的鼠李糖乳杆菌LGG显示出更好的抑制所有测试的病原体粘附到猪肠粘液的能力。益生菌组合可用于抵消肠道微生物群中与疾病相关的畸变。针对特定的猪病原体，可以选择特定的保护性益生菌。来自人源的益生菌菌株和预期用于人的益生菌菌株也粘附于猪肠粘液，并且能够置换和抑制病原体。
The aim of this study was to investigate in vitro the protective effect of commercial probiotic strains (Bifidobacterium lactis Bb12 and Lactobacillus rhamnosus LGG) alone and in combination on the adhesion of pathogenic strains as Salmonella, Clostridium, and Escherichia coli to pig intestinal mucus obtained from different intestinal regions. In combination, probiotic strains enhanced each other's adhesion, mainly in large intestinal mucus. Treatment of intestinal mucus with Bb12 and LGG, alone or in combination, significantly reduced (P < 0.05) the adhesion of the tested pathogens. The ability to inhibit pathogen adhesion appears to depend on the specific probiotics and pathogens and on the mucosal site. B. lactis Bb12 and L. rhamnosus LGG in combination revealed a better ability to inhibit adhesion of all pathogens tested to pig intestinal mucus than probiotic strains. Probiotic combinations could be useful for counteracting disease-associated aberrations in intestinal microbiota. Specific protective probiotics could be selected for particular pig pathogens. Probiotic strains from human origin and intended for human use also adhere to pig intestinal mucus and are able to displace and inhibit pathogens.