Mechanism for membrane electroporation irreversibility under high-intensity, ultrashort electrical pulse conditions
Mechanism for membrane electroporation irreversibility under high-intensity, ultrashort electrical pulse conditions
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DOI：
10.1103/physreve.66.052901
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发表时间：
2002-11-01
影响因子：
2.4
通讯作者：
Schoenbach, KH
中科院分区：
文献类型：
--
作者：
Joshi, RP;Schoenbach, KH
使用改进的电穿孔模型来解决超短电脉冲条件下膜的不可逆性。它表明，膜可以生存的强电脉冲和恢复提供的孔分布有一个相对较大的蔓延。然而，如果群体主要由较大半径的孔组成，则可能导致不可逆性。从物理上讲，如果相邻部位的孔隙合并，可能会出现这种分布。在较小的细胞器中比在外部细胞膜中更容易满足孔位点之间紧密接近的要求。模型预测与最近对细胞内细胞器的细胞损伤的观察一致（例如，线粒体），没有不可逆的冲击外膜，由纳秒，高强度的电脉冲。这种机制也解释了多次电击造成的更大伤害。
An improved electroporation model is used to address membrane irreversibility under ultrashort electric pulse conditions. It is shown that membranes can survive a strong electric pulse and recover provided the pore distribution has a relatively large spread. If, however, the population consists predominantly of larger radii pores, then irreversibility can result. Physically, such a distribution could arise if pores at adjacent sites coalesce. The requirement of close proximity among the pore sites is more easily satisfied in smaller organelles than in outer cell membranes. Model predictions are in keeping with recent observations of cell damage to intracellular organelles (e.g., mitochondria), without irreversible shock at the outer membranes, by a nanosecond, high-intensity electric pulse. This mechanism also explains the greater damage from multiple electric shocks.