Contribution to deterioration of polymeric materials by a slow-growing bacterium Nocardia corynebacterioides
Contribution to deterioration of polymeric materials by a slow-growing bacterium Nocardia corynebacterioides
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DOI：
10.1016/j.ibiod.2008.06.003
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发表时间：
2009-01-01
影响因子：
4.8
通讯作者：
Cheng, Shu-Pei
中科院分区：
文献类型：
--
作者：
Pan, Li;Gu, Ji-Guang;Cheng, Shu-Pei
能够降解聚合物产品的细菌从几种来源分离出来，包括自来水、沉积物和变质的聚合物产品。产碱杆菌属xylosoxidans;T2、铜绿假单胞菌GP10和类棒杆菌诺卡菌S3能够利用橡胶制品作为碳和能量的唯一来源。这些微生物在添加橡胶条和橡胶提取物的无机盐培养基中表现出不同的生长模式。与橡胶制品在MSM中孵育70天后，木糖氧化A. T2、铜绿假单胞菌GP10和棒状芽孢杆菌S3分别使橡胶制品的重量减少了约2.0、4.0和5.3%。在培养7天后，平均有0.45 mg（水溶性碳）g（-1）的橡胶制品释放到溶液中。N.棒状拟杆菌S3最初生长较慢，但在聚合物产物定植后超过了其他两种细菌。扫描电镜观察到，拟棒状芽孢杆菌S3在橡胶制品表面形成致密的生物膜。这项研究表明，在一系列环境中的本地微生物能够通过利用从聚合物基质中释放的化学物质来降解聚合物产物，但材料表面生长缓慢的微生物在材料变质中起着更明显的作用。(c) 2008 Elsevier Ltd.版权所有。
Bacteria capable of degrading polymeric products were isolated from several sources including tap water, sediment, and a deteriorated polymeric product. Alcaligenes xylosoxidans; T2, Pseudomonas aeruginosa GP10, and Nocardia corynebacterioides S3 were able to utilize rubber products as a sole source of carbon and energy. These microorganisms showed different growth patterns in mineral salt media (MSM) supplemented with rubber strips or with the rubber extract. A. xylosoxidans T2, P. aeruginosa GP10 and N. corynebacterioides S3 reduced the weight of the rubber product by approximately 2.0, 4.0 and 5.3%, respectively, after 70 days of incubation with the rubber product in MSM. On average, 0.45 mg (water-soluble carbon) g(-1) of the rubber product was released into solution phase after 7 days of incubation. Growth of N. corynebacterloides S3 was initially slower but exceeded the other two bacteria upon colonization of the polymer products. N. corynebacterioides S3 formed a dense biofilm on surfaces of the rubber product as observed under scanning electron microscopy. This study suggests that indigenous microorganisms in a range of environments are capable of degrading polymeric products through utilization of chemicals released from the polymer matrix, but the slow-growing microorganisms on material surfaces have a much more pronounced role in the materials deterioration. (c) 2008 Elsevier Ltd. All rights reserved.