Mandarin peelings:: The best carbon source to produce laccase by static cultures of Trametes pubescens
Mandarin peelings:: The best carbon source to produce laccase by static cultures of Trametes pubescens
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DOI：
10.1016/j.chemosphere.2006.11.051
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发表时间：
2007-04-01
期刊：
影响因子：
8.8
通讯作者：
Couto, Susana Rodriguez
中科院分区：
文献类型：
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作者：
Osma, Johann F.;Saravia, Vernica;Couto, Susana Rodriguez
在本研究中，我们研究了不同的碳源（葡萄糖，甘油和地面柑橘皮）的影响，漆酶的毛栓菌生长在不锈钢海绵在静态条件下。以柑橘皮为基质的培养物漆酶活性最高，约为100 U 1（-1）。这是一个非常有趣的结果，因为柑橘皮在一些地区，如地中海和亚洲国家是常见的农业废物。因此，它们的再利用除了降低培养基成本外，还有助于解决其处置带来的污染问题。此外，我们还研究了在培养基中添加不同潜在的漆酶诱导化合物（ABTS、吐温20、大豆油、马来绿色、Cu 2+、单宁酸）对漆酶产量的影响。大豆油是漆酶活性的最佳诱导剂，其值比参考培养物中获得的值高4倍。(c)2006爱思唯尔有限公司保留所有权利。
In the present study, we investigated the effect of different carbon sources (glucose, glycerol and ground mandarin peelings) on laccase production by Trametes pubescens grown on stainless steel sponges under static conditions. The cultures with ground mandarin peelings gave the highest laccase activities, showing values of about 100 U 1(-1). This is a very interesting result, since mandarin peelings are common agricultural wastes in some regions such as Mediterranean and Asiatic countries. Therefore, their reutilisation, besides reducing medium cost, also helps to solve the pollution problems caused by their disposal.Also, we studied the effect of supplementing the culture medium with different potential laccase-inducing compounds (ABTS, Tween 20, soya oil, Malaquite Green, Cu2+, tannic acid) on laccase production. Soya oil was the best inducer of laccase activities, attaining values 4-fold higher than those obtained in the reference cultures. (c) 2006 Elsevier Ltd. All rights reserved.