Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains
Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains
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DOI：
10.1038/s41598-019-40922-x
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发表时间：
2019-03-14
影响因子：
4.6
通讯作者：
Kirsebom, Leif A.
中科院分区：
文献类型：
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作者：
Behra, Phani Rama Krishna;Das, Sarbashis;Kirsebom, Leif A.
龟分枝杆菌复合体（MCAC）的成员与分枝杆菌的祖先很接近，包括人类、动物和鱼类病原体。我们提出了这个复合体的14个成员的基因组：沙门氏分枝杆菌和龟分枝杆菌型菌株的完整基因组，7个M。salmoniphilum分离物和5株M.沙门氏菌样菌株，包括在乌普萨拉大学的动物设施中爆发期间分离的菌株。平均核苷酸同源性分析和核心基因同源性分析表明，M.沙门氏菌样菌株是人类病原体Franklinii分枝杆菌的变体，并且在遗传学上接近于Mycobacteriumsessus。我们的数据进一步表明M. salmoniphilum分为三个分支，分别命名为类群I、II和III。属于组II的嗜沙门氏菌型菌株。在预测的毒力因子中，磷脂酶C（plcC）的存在，这是一个主要的毒力因子，使M。对小鼠巨噬细胞有较强的细胞毒活性; franklinii最初是从受感染的人身上分离出来的，这使得动物设施中的爆发是由M.沙门氏菌样菌株。有趣的是，M。从自来水中分离出类沙门氏菌，表明它可以存在于环境中。此外，我们预测了突变热点的存在，在M。嗜鲑鱼分离株和其中26%的热点与被归类为在毒力、疾病和防御中发挥作用的基因重叠。我们还提供了有关转录和翻译的关键基因，如σ因子，核糖体蛋白和tRNA基因的数据。
Members of the Mycobacterium chelonae-abscessus complex (MCAC) are close to the mycobacterial ancestor and includes both human, animal and fish pathogens. We present the genomes of 14 members of this complex: the complete genomes of Mycobacterium salmoniphilum and Mycobacterium chelonae type strains, seven M. salmoniphilum isolates, and five M. salmoniphilum-like strains including strains isolated during an outbreak in an animal facility at Uppsala University. Average nucleotide identity (ANI) analysis and core gene phylogeny revealed that the M. salmoniphilum-like strains are variants of the human pathogen Mycobacterium franklinii and phylogenetically close to Mycobacterium abscessus. Our data further suggested that M. salmoniphilum separates into three branches named group I, II and III with the M. salmoniphilum type strain belonging to group II. Among predicted virulence factors, the presence of phospholipase C (plcC), which is a major virulence factor that makes M. abscessus highly cytotoxic to mouse macrophages, and that M. franklinii originally was isolated from infected humans make it plausible that the outbreak in the animal facility was caused by a M. salmoniphilum-like strain. Interestingly, M. salmoniphilum-like was isolated from tap water suggesting that it can be present in the environment. Moreover, we predicted the presence of mutational hotspots in the M. salmoniphilum isolates and 26% of these hotspots overlap with genes categorized as having roles in virulence, disease and defense. We also provide data about key genes involved in transcription and translation such as sigma factor, ribosomal protein and tRNA genes.