Bisphenol A promotes the proliferation of leiomyoma cells by GPR30-EGFR signaling pathway
Bisphenol A promotes the proliferation of leiomyoma cells by GPR30-EGFR signaling pathway
复制标题
双酚A通过GPR30-EGFR信号通路促进平滑肌瘤细胞增殖
DOI：
10.1111/jog.13972
复制
发表时间：
2019-07-01
影响因子：
1.6
通讯作者：
Shen, Yang
中科院分区：
文献类型：
--
作者：
Li, Zemin;Lu, Qing;Shen, Yang
目的探讨G蛋白偶联受体30-表皮生长因子受体（GPR30-EGFR）信号通路对双酚a诱导的子宫平滑肌瘤细胞增殖的分子机制。采用实时定量聚合酶链反应法和Western blot法检测15例及各组肌瘤组织标本中GPR30和EGFR信使RNA及蛋白的表达。Western blot检测丝裂原活化蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)/c-fos信号通路成员的蛋白表达。结果双酚A能促进UL细胞的生长和GPR30、EGFR、c-fos、p-ERK1/2的表达。结论双酚A可通过激活GPR30-EGFR和MAPK/ERK/c-fos信号通路成员的转录和翻译，特异性激活人UL细胞增殖。
Aim To study the molecular mechanism of G protein-coupled receptor 30-epidermal growth factor receptor (GPR30-EGFR) signaling pathway on the proliferation of leiomyoma cells exposed with bisphenol A. Methods Primary cultures and subcultures of human uterine leiomyoma (UL) cells. The expressions of messenger RNA and proteins of GPR30 and EGFR in 15 leiomyoma tissue specimens and all groups were detected by real-time quantitative polymerase chain reaction assay and Western blot assay. The protein of mitogen-activated protein kinases (MAPK)/extracellular signal-regulated kinases (ERK)/c-fos signaling pathway members was detected by Western blot assay. Results Bisphenol A promoted the growth of UL cells and the expressions of GPR30, EGFR, c-fos and p-ERK1/2. Conclusion Bisphenol A was found to be a promoter specifically to proliferate the human UL cells by activating the transcription and translation of GPR30-EGFR and MAPK/ERK/c-fos signaling pathway members.