Culex pipiens (Diptera: Culicidae):: A bridge vector of West Nile virus to humans
Culex pipiens (Diptera: Culicidae):: A bridge vector of West Nile virus to humans
复制标题
DOI：
10.1603/0022-2585(2008)45
复制
发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Walker, Edward D.
中科院分区：
文献类型：
--
作者：
Hamer, Gabriel L.;Kitron, Uriel D.;Walker, Edward D.
尖音库蚊的寄主取食方式2005年在芝加哥西南郊区采集的20株猪流感病毒，采用聚合酶链反应（PCR）和DNA测序技术进行了研究。库蚊属蚊子，大多数被鉴定为Cx。pipiens和其余的Cx。restuans通过PCR，已经以18种鸟类为食，最常见的是美国知更鸟（Turdus migratorious），家雀（Passer migraticus）和哀鸠（Zenaida macroura）。另外的血粉来自四种哺乳动物，主要是人类和浣熊（Procyon lotor）。在2005年西尼罗河病毒（WNV）流行期间，在5只Cx的珠和胸中检测到西尼罗河病毒（WNV）RNA。pipiens（n = 335，1.5%）。这些病毒感染的吸血蚊子的宿主包括两只美国知更鸟，一只家雀和一个人。这是首例WNV感染Cx的报告。在西尼罗河病毒流行期间收集的尖音蚊被发现叮咬了一个人。这些结果满足Cx的定罪标准。pipiens作为桥梁矢量。
Host-feeding patterns of Culex pipiens L. collected in southwest suburban Chicago in 2005 were studied using polymerase chain reaction (PCR) and DNA sequencing techniques. Culex spp. mosquitoes, most identified to Cx. pipiens and the remainder to Cx. restuans by PCR, had fed on 18 avian species, most commonly American robin (Turdus migratorious), house sparrow (Passer domesticus), and mourning dove (Zenaida macroura). Additional blood meals were derived from four mammal species, primarily humans and raccoons (Procyon lotor). During a West Nile virus (WNV) epidemic in 2005, West Nile virus (WNV) RNA was detected in beads and thoraces of five Cx. pipiens (n = 335, 1.5%) using quantitative PCR. The hosts of these virus-infected, blood-fed mosquitoes included two American robins, one house sparrow, and one human. This is the first report of a WNV-infected Cx. pipiens mosquito collected during an epidemic of WNV that was found to have bitten a human. These results fulfill a criterion for incrimination of Cx. pipiens as a bridge vector.