THE TOXIGENIC ELEMENT OF CLOSTRIDIUM-DIFFICILE STRAIN VPI-10463
THE TOXIGENIC ELEMENT OF CLOSTRIDIUM-DIFFICILE STRAIN VPI-10463
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DOI：
10.1016/s0882-4010(95)90263-5
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发表时间：
1995-10-01
影响因子：
3.8
通讯作者：
JOHNSON, JL
中科院分区：
文献类型：
--
作者：
HAMMOND, GA;JOHNSON, JL
艰难梭菌菌株VPI 10463的转基因元件通过在转基因序列与非转基因和转基因菌株共有的那些序列之间建立边界来鉴定。该基因元件是染色体的，长19.6 kb，由5个开放阅读框组成，其中包括毒素A和B基因。四个开放阅读框是连续的，并且以相同的方向转录。第五个是下游的其他和面向相反的方向。其中一个开放阅读框位于毒素B基因的5'端，以前是未知的。在3个无毒菌株中，一个127bp的短片段与19.6kb的大片段位于同一染色体位置。(C)1995年学术出版社
The toxigenic element of Clostridium difficile strain VPI 10463 is identified by establishing boundaries between toxigenic sequences and those sequences shared by nontoxigenic and toxigenic strains. The toxigenic element is chromosomal, 19.6 kb in length, and comprised of five open reading frames which include the toxin A and B genes. Four of the open reading frames are contiguous and are transcribed in the same direction. The fifth is downstream from the others and oriented in the opposite direction. One of the open reading frames, located 5' to the toxin B gene, is previously unknown.Both upstream (5') and downstream (3') boundaries for the toxigenic element were examined in six toxigenic strains which vary considerably in toxigenicity to determine if there were variations among their respective toxigenic elements. The toxigenic element is highly conserved in these six strains.In the three nontoxignic strains examined, a short fragment (127 bp) occupies the same chromosomal location as the large 19.6 kb toxigenic element. (C) 1995 Academic Press Limited