The culture of pig oocytes in minimal medium, and the influence of progesterone and estradiol-17beta on meiotic maturation.
The culture of pig oocytes in minimal medium, and the influence of progesterone and estradiol-17beta on meiotic maturation.
复制标题
猪卵母细胞在基本培养基中的培养，以及孕酮和雌二醇-17β对减数分裂成熟的影响。
DOI：
10.1210/endo-100-1-39
复制
发表时间：
1977
期刊：
影响因子：
4.8
通讯作者：
R. McGaughey
中科院分区：
文献类型：
--
作者：
R. McGaughey
猪卵母细胞，（完整）或无（裸露）粘附颗粒细胞，培养33至35小时，在最低限度的培养基中含有牛血清白蛋白和孕酮或雌二醇-17 β或类固醇。完整卵母细胞在基本培养基中成熟的发生率与其他研究者使用复合培养基观察到的结果相当，如果不是上级的话。无论是完整的还是裸露的卵母细胞，孕酮对成熟的刺激都没有统计学意义。雌二醇-17 β在浓度为1.0和10.0 μ g/ml的抑制裸露的卵母细胞的成熟，但抑制被克服，包括孕酮在培养基中，或随后培养的抑制的卵母细胞中的类固醇自由的培养基。空气干燥的卵母细胞的染色体检查表明，完整的和裸露的卵母细胞培养在孕酮或雌二醇-17 β表现出增加的发病率的二倍体和超单倍体在末期I和中期II，但培养在培养基中含有孕酮和雌二醇的卵母细胞显示出较高的发病率正常的单倍体比对照卵母细胞。
Pig oocytes, with (intact) or without (denuded) adhering granulosa cells, were cultured for 33 to 35 h in minimal culture media containing bovine serum albumin and either progesterone or estradiol-17beta or both steroids. The incidence of maturation for intact oocytes in minimal medium was comparable, if not superior, to that observed by other investigators using complex culture media. No statistically significant stimulation of maturation by progesterone was observed for either intact or denuded oocytes. Estradiol-17beta at concentrations of 1.0 and 10.0 mug per ml inhibited the maturation of denuded oocytes, but the inhibition was overcome either by including progesterone in the medium or by subsequently culturing the inhibited oocytes in steriod-free medium. Chromosomal examination of air-dried oocytes indicated that intact and denuded oocytes cultured either in progesterone or estradiol-17beta exhibited an increased incidence of diploidy and hyperhaploidy at telophase I and metaphase II, but that oocytes cultured in media containing both progesterone and estradiol showed a higher incidence of normal haploidy than did control oocytes.