Genetic labeling of mouse rhombomeres by Cadherin-6::EGFP-BAC transgenesis underscores the role of cadherins in hindbrain compartmentalization
Genetic labeling of mouse rhombomeres by Cadherin-6::EGFP-BAC transgenesis underscores the role of cadherins in hindbrain compartmentalization
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DOI：
10.1016/j.neures.2008.09.005
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Inoue, Takayoshi
中科院分区：
文献类型：
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作者：
Inoue, Yukiko U.;Asami, Junko;Inoue, Takayoshi
Cadherin-6 (Cdh6) 是一种 11 型经典钙粘蛋白细胞粘附分子，其表达描绘了小鼠早期发育过程中特定的菱形体组。在这里，我们建立了一个稳定的 BAC 转基因小鼠系，其中增强的绿色荧光蛋白 (EGFP) 重现了胚胎后脑内的 Cdh6 表达。当在胚胎（E）第8.75-E9.75天将含有EGFP阳性菱形细胞（r）6-7和EGFP阴性r4-5细胞的转基因后后脑区域解离为单细胞并在悬浮液中孵育时，发现它们仅在钙离子存在下才形成聚集体，并且EGFP-Cdh6阳性/阴性群体在聚集体中彼此分离。我们进一步证明，通过允许 Hox 基因表达在整个胚胎培养系统中前移的处理，E9.5 处转基因后脑中的 EGFP-Cdh6 表达边界向喙侧移动。这些结果表明，Cdh6 在协调小鼠后脑区室中的作用可能是在 Hox 基因调控网络的控制下。 (C) 2008 Elsevier Ireland Ltd 和日本神经科学学会。版权所有。
Cadherin-6(Cdh6) is a type 11 classic cadherin cell adhesion molecule whose expression delineates specific sets of rhombomeres during early mouse development. Here we establish a stable BAC transgenic mouse line in which enhanced green fluorescent protein (EGFP) recapitulates Cdh6 expression within the embryonic hindbrain. When the transgenic posterior hindbrain region at the embryonic (E) day 8.75-E9.75 that contains EGFP-positive rhombomere (r) 6-7 and EGFP-negative r4-5 cells was dissociated to single cells and incubated in suspensions, they were found to make aggregates only in the presence of calcium ion with the EGFP-Cdh6-positive/negative populations segregating each other in an aggregate. We further demonstrated that EGFP-Cdh6 expression boundary in the transgenic hindbrain at E9.5 shifted rostrally by the treatment that allows Hox gene expression anteriorize in the whole embryo Culture system. These results suggest the role of Cdh6 in coordinating mouse hindbrain compartments possibly under the control of Hox gene regulatory network. (C) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.