Determination of retinoic acid (13-cis- and all-trans-) and aromatic retinoic acid analogs possessing anti-tumor activity, in biological fluids by high-performance liquid chromatography.
Determination of retinoic acid (13-cis- and all-trans-) and aromatic retinoic acid analogs possessing anti-tumor activity, in biological fluids by high-performance liquid chromatography.
复制标题
采用高效液相色谱法测定生物体液中具有抗肿瘤活性的视黄酸（13-顺式和全反式）和芳香族视黄酸类似物。
DOI：
--
复制
发表时间：
1978
影响因子：
4.1
通讯作者：
J. D. de Silva
中科院分区：
文献类型：
--
作者：
C. Puglisi;J. D. de Silva
建立了血或尿中13-顺式和全反式维甲酸的高效液相色谱检测方法，总回收率为90+/-5.0%，检出限为10-20 ng/ml。该方法提供了使用1毫升血液的快速和简单的化合物定量。该方法用于测定犬静脉和口服剂量分别为9.5 mg/kg和2.0 mg/kg的13-顺式维甲酸的血药浓度，以及单次口服100 mg剂量和每日分次给药共2 mg/kg体重后的血中13-顺式维甲酸浓度。该方法也适用于一系列具有临床意义的芳香族维甲酸类似物作为抗肿瘤药物的测定。
A sensitive and specific high-performance liquid chromatographic assay was developed for the determination of 13-cis- and all-trans-retinoic acid in blood or urine with an overall recovery of 90 +/- 5.0% and a limit of detection of 10-20 ng/ml of sample. The method provides for rapid and simple quantitation of the compounds using 1 ml of blood. The assay was applied in the determination of blood levels of 13-cis-retinoic acid in the dog following intravenous and oral administration of 9.5 mg/kg and 2.0 mg/kg doses, and in man following a single 100-mg oral dose and following divided daily doses totalling 2 mg per kg of body weight. The assay is also applicable with minor modifications to the determination of a series of aromatic retinoic acid analogs of clinical interest as anti-tumor agents.