In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly(D,L-lactide) scaffold fabricated by cryogenic electrospinning technique
In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly(D,L-lactide) scaffold fabricated by cryogenic electrospinning technique
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DOI：
10.1002/jbm.a.32208
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发表时间：
2009-10-01
影响因子：
4.9
通讯作者：
Chian, Kerm Sin
中科院分区：
文献类型：
--
作者：
Leong, Meng Fatt;Rasheed, Mohamed Zulfikar;Chian, Kerm Sin
限制电纺支架应用于组织工程的障碍之一是抑制细胞浸润的纳米级孔。在这篇文章中，我们描述了一种技术，使用冰晶作为模板制造低温静电纺丝支架（CES）与大的三维和互连孔使用聚（D，L-丙交酯）（PLA）。通过控制静电纺丝环境的湿度，可以控制孔径。根据所使用的相对湿度，我们能够实现从900 +/- 100 μ m（2）到5000 +/- 2000 μ m（2）的孔径范围。我们的研究结果表明，在静态培养条件下，细胞渗透的CES高达50 μ m的厚度在体外，而细胞没有渗透到传统的静电纺丝支架。体内研究表明，与常规制备的静电纺丝支架相比，CES中的细胞浸润和血管化得到改善。在获得对孔特征的控制时，我们可以设计针对特定组织工程应用优化的CES。(C)2008 Wiley Periodicals，Inc. J Biomed Mater Res 91A：231-240，2009
One of the obstacles limiting the application of electrospun scaffolds for tissue engineering is the nanoscale pores that inhibit cell infiltration. In this article, we describe a technique that uses ice crystals as templates to fabricate cryogenic electrospun scaffolds (CES) with large three-dimensional and interconnected pores using poly(D,L-lactide) (PLA). Manipulating the humidity of the electrospinning environment the pore sizes are controlled. We are able to achieve pore sizes ranging from 900 +/- 100 mu m(2) to 5000 +/- 2000 mu m(2) depending on the relative humidity used. Our results show that cells infiltrated the CES up to 50 mu m in thickness in vitro under static culture conditions whereas cells did not infiltrate the conventional electrospun scaffolds. In vivo studies demonstrated improved cell infiltration and vascularization in the CES compared with conventionally prepared electrospun scaffolds. In gaining control of the pore characteristics, we can then design CES that are optimized for specific tissue engineering applications. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 231-240, 2009