Genome-Wide Analysis of Watermelon HSP20s and Their Expression Profiles and Subcellular Locations under Stresses
Genome-Wide Analysis of Watermelon HSP20s and Their Expression Profiles and Subcellular Locations under Stresses
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西瓜 HSP20 及其表达谱和应激下亚细胞位置的全基因组分析
DOI：
10.3390/ijms20010012
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发表时间：
2019-01-01
影响因子：
5.6
通讯作者：
Li, Lili
中科院分区：
文献类型：
--
作者：
He, Yanjun;Fan, Min;Li, Lili
西瓜（Citrullus lanatus L.），葫芦科作物是世界范围内种植的经济上重要的葫芦科作物，易受各种不利环境条件的影响。小分子热休克蛋白20 s（HSP 20 s）是植物中含量最丰富的热休克蛋白，在各种生物和非生物胁迫反应中发挥重要作用。然而，他们还没有在西瓜进行系统的调查。本研究共鉴定了44个西瓜HSP 20基因，并对其基因结构、保守结构域、系统发育关系、染色体分布和表达谱进行了分析。所有西瓜HSP 20蛋白都具有保守的α-晶体蛋白（ACD）结构域。半数ClHSP 20通过基因复制事件产生。本研究将植物HSP 20分为18个亚家族，并鉴定了一个新的亚家族--核质XIII（CXIII）。在西瓜HSP 20基因的启动子区域检测到许多胁迫和胁迫响应的顺式元件。与其他物种不同的是，西瓜中有一半的HSP 20受到热胁迫的抑制。植物HSP 20对不同病毒感染表现出不同的反应，大多数ClHSP 20通常被黄瓜绿色斑驳花叶病毒（CGMMV）抑制。一些ClHSP 20对脱落酸、褪黑激素和CGMMV表现出类似的转录反应。6个选择的HSP 20-绿色荧光蛋白融合蛋白的亚细胞定位分析揭示了不同的亚细胞靶向。一些ClHSP 20蛋白受到CGMMV的影响，反映了荧光颗粒的大小，数量和分布的变化。这些系统分析为阐明西瓜HSP 20基因家族的生理功能和生物学作用奠定了基础。
Watermelon (Citrullus lanatus L.), which is an economically important cucurbit crop that is cultivated worldwide, is vulnerable to various adverse environmental conditions. Small heat shock protein 20s (HSP20s) are the most abundant plant HSPs and they play important roles in various biotic and abiotic stress responses. However, they have not been systematically investigated in watermelon. In this study, we identified 44 watermelon HSP20 genes and analyzed their gene structures, conserved domains, phylogenetic relationships, chromosomal distributions, and expression profiles. All of the watermelon HSP20 proteins have a conserved the α-crystallin (ACD) domain. Half of the ClHSP20s arose through gene duplication events. Plant HSP20s were grouped into 18 subfamiles and a new subfamily, nucleo-cytoplasmic XIII (CXIII), was identified in this study. Numerous stress- and hormone-responsive cis-elements were detected in the putative promoter regions of the watermelon HSP20 genes. Different from that in other species, half of the watermelon HSP20s were repressed by heat stress. Plant HSP20s displayed diverse responses to different virus infections and most of the ClHSP20s were generally repressed by Cucumber green mottle mosaic virus (CGMMV). Some ClHSP20s exhibited similar transcriptional responses to abscisic acid, melatonin, and CGMMV. Subcellular localization analyses of six selected HSP20- green fluorescence protein fusion proteins revealed diverse subcellular targeting. Some ClHSP20 proteins were affected by CGMMV, as reflected by changes in the size, number, and distribution of fluorescent granules. These systematic analyses provide a foundation for elucidating the physiological functions and biological roles of the watermelon HSP20 gene family.