Single-walled carbon nanotube induces oxidative stress and activates nuclear transcription factor-κB in human keratinocytes
Single-walled carbon nanotube induces oxidative stress and activates nuclear transcription factor-κB in human keratinocytes
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DOI：
10.1021/nl0507966
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发表时间：
2005-09-01
期刊：
影响因子：
10.8
通讯作者：
Ramesh, GT
中科院分区：
文献类型：
--
作者：
Manna, SK;Sarkar, S;Ramesh, GT
碳纳米管由于其独特的物理性质，现在正成为日常生活中使用的重要材料。尚未详细研究这些材料的毒理学影响，因此限制了其使用。在本研究中，单壁碳纳米管（SWCNT）的毒性在人类角质形成细胞进行了评估。结果显示，响应于用SWCNT颗粒处理角质形成细胞，氧化应激增加和细胞增殖抑制。此外，研究了暴露于SWCNT颗粒后角质形成细胞中的信号传导机制。研究结果表明，SWCNT颗粒在人角质形成细胞中以剂量依赖性方式激活NF-κ B。此外，NF-κ B的活化机制是由于角质形成细胞中SWCNT颗粒对应激相关激酶的活化。总之，这些研究显示了单壁碳纳米管颗粒诱导的毒性机制。
Carbon nanotubes are now becoming an important material for use in day to day life because of their unique physical properties. The toxicological impact of these materials has not yet been studied in detail, thereby limiting their use. In the present study, the toxicity of single-walled carbon nanotubes (SWCNT) was assessed in human keratinocyte cells. The results show increased oxidative stress and inhibition of cell proliferation in response to treatment of keratinocytes with SWCNT particles. In addition, the signaling mechanism in keratinocytes upon exposure to SWCNT particles was investigated. Results from the study suggest that SWCNT particles activate NF-kappa B in a dose-dependent manner in human keratinocytes. Further, the mechanism of activation of NF-kappa B was due to the activation of stress-related kinases by SWCNT particles in keratinocytes. In conclusion, these studies show the mechanism of toxicity induced by SWCNT particles.