DISRUPTION OF THE GENE ENCODING THE NADH-BINDING SUBUNIT OF NADH-UBIQUINONE OXIDOREDUCTASE IN NEUROSPORA-CRASSA FORMATION OF A PARTIALLY ASSEMBLED ENZYME WITHOUT FMN AND THE IRON-SULFUR CLUSTER N-3
DISRUPTION OF THE GENE ENCODING THE NADH-BINDING SUBUNIT OF NADH-UBIQUINONE OXIDOREDUCTASE IN NEUROSPORA-CRASSA FORMATION OF A PARTIALLY ASSEMBLED ENZYME WITHOUT FMN AND THE IRON-SULFUR CLUSTER N-3
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DOI：
10.1111/j.1432-1033.1994.tb18655.x
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发表时间：
1994-03-01
期刊：
影响因子：
--
通讯作者：
WEISS, H
中科院分区：
文献类型：
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作者：
FECKE, W;SLED, VD;WEISS, H
在本研究中，粗神经孢子虫线粒体NADH的51-kDa NADH结合亚基：泛醌氧化还原酶（复合体I）的基因被同源替换为缺陷基因拷贝而失活。由此产生的突变体nuo51缺乏51-kDa亚基，没有复合物I活性，但仍以野生型生长速度的三分之一生长。替代NADH：泛醌氧化还原酶(s)的酶活性增加了两倍，而其他线粒体呼吸酶的活性正常。除了nadh结合亚基外，配合物I几乎完全组装，并且仍然具有epr可检测到的四个铁硫簇中的三个。由于缺失的亚基包含一个四核铁硫簇的序列基序，因此缺失的簇N-3被认为与该亚基结合。
In this study, the gene of the 51-kDa NADH-binding subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I) in Neurospora crassa was inactivated by homologous replacement with a defective gene copy. The resulting mutant, nuo51, lacks the 51-kDa subunit and shows no complex I activity but still grows at one third of the wild-type growth rate. The enzyme activity of the alternative NADH:ubiquinone oxidoreductase(s) is increased twofold while the activities of the other mitochondrial respiratory enzymes are normal. Complex I is almost completely assembled except for the NADH-binding subunit and still possesses three out of the four EPR-detectable iron-sulphur clusters. Since the deleted subunit contains the sequence motif for one tetranuclear iron-sulphur cluster, the missing cluster N-3 is considered to be bound to this subunit.