PATHWAYS OF LYMPHOCYTE MIGRATION WITHIN THE PERIARTERIAL LYMPHOID SHEATH OF RAT SPLEEN
PATHWAYS OF LYMPHOCYTE MIGRATION WITHIN THE PERIARTERIAL LYMPHOID SHEATH OF RAT SPLEEN
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DOI：
10.1007/bf00217236
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发表时间：
1984-01-01
影响因子：
3.6
通讯作者：
REISERT, I
中科院分区：
文献类型：
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作者：
BRELINSKA, R;PILGRIM, C;REISERT, I
雄性Wistar大鼠静脉注射。用5-(~3H)尿苷标记的淋巴细胞从同基因供者的淋巴中分离并富含T细胞。短时间后(注射后3~120min)，用放射自显影将标记淋巴细胞定位于脾实质，以确定淋巴细胞从血液进入脾实质的路径，并跟踪循环淋巴管在动脉周围淋巴鞘(PALS)内的迁移路径。以动脉直径为特征的PALS标记淋巴细胞在特定平面上进行分析，PALS分为大、中、小三种类型(分别为PALS L、PALS M、PALS S)。还注意到靠近动脉血管壁的标记淋巴细胞的堆积。标记淋巴细胞最初定位于PAL S和PALS M，位于动脉末梢分支附近的边缘带(MZ)。淋巴细胞明显地从血液中迁移到脾实质中的两个白髓室：在MZ，和直接在PAL内通过毛细血管的壁。标记细胞在直径增大的动脉血管附近的顺序聚集表明，淋巴细胞再循环池通过两条途径迁移到PALS L的中央部分：来自MZ的，以及来自PALS S和PALS M的沿动脉血管的迁移。
Male Wistar rats were injected i.v. with 5-(3H)uridine-labeled lymphocytes isolated from lymph nodes of syngeneic donors and enriched in T cells. After short periods of time (3-120 min after injection), labeled lymphocytes were localized in spleen compartments using autoradiography to identify routes of lymphocyte movement from blood into splenic parenchyma and to follow migration pathways of recirculating lymphoctyes within the periarterial lymphoid sheath (PALS). Topographical analysis of labeled lymphocytes was performed in specific planes of PALS characterized by the diameter of the arterial vessel and termed PALS large, PALS medium, and PALS small (PALS L, PALS M, PALS S, respectively). Attention was also paid to accumulations of labeled lymphocytes close to the arterial vessel wall. Initially, labeled lymphocytes were localized in PALS S and PALS M near the terminal branching of arterial vessels and in the marginal zone (MZ). Lymphocytes apparently emigrate from blood into splenic parenchyma within 2 white pulp compartments: in MZ, and directly within PALS through the wall of capillary vessels. The sequential accumulation of labeled cells near arterial vessels of increasing diameter suggests that the recirculating pool of lymphocytes migrates into the central part of PALS L by 2 routes: from MZ, and along arterial vessels from PALS S and PALS M.